Department of Materials Science and Engineering, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Department of Orthodontics, Faculty of Dentistry, Mahidol University, Bangkok, 10400, Thailand.
Arch Oral Biol. 2021 Mar;123:105041. doi: 10.1016/j.archoralbio.2021.105041. Epub 2021 Jan 8.
The aim of this study was to develop a composite scaffold with the optimal poly(lactic-co-glycolic acid) (PLGA) and bioactive glass proportions to provide an environment for bone tissue regeneration and repair.
PLGA-bioactive glass composite scaffolds were prepared using a salt-leaching technique with different percentages of bioactive glass (0%, 10 %, and 15 % [w/w]) with PLGA. The resulting scaffolds were characterized using scanning electron microscopy and energy dispersive X-ray spectroscopy (SEM-EDS), and water contact angle, dynamic mechanical, and pH analysis. The scaffold biocompatibility was investigated using stem cells from human exfoliated deciduous teeth (SHED) and rat experiments.
SEM-EDS confirmed the successful fabrication of three-dimensional PLGA-bioactive glass scaffolds. The results showed that 10 % bioactive glass with PLGA exhibited favorable properties including increased pore size, hydrophilicity, and mechanical properties. The growth medium pH was increased for scaffolds containing bioactive glass. All scaffolds were biocompatible, and 10 % bioactive glass composite scaffolding showed better attachment, growth, and proliferation of SHED compared to the other scaffolds. Moreover, it enhanced osteogenic differentiation of SHED in vitro and in vivo.
Salt-leaching-derived PLGA-bioactive glass composite scaffolds were successfully established. PLGA with 10 % bioactive glass had adequate physical properties and bioactivity, and it could be considered as a composite for bone tissue engineering applications.
本研究旨在开发一种具有最佳聚（乳酸-共-乙醇酸）（PLGA）和生物活性玻璃比例的复合支架，为骨组织再生和修复提供环境。
采用盐浸法制备 PLGA-生物活性玻璃复合支架，PLGA 中分别含有 0%、10%和 15%（w/w）的生物活性玻璃。采用扫描电子显微镜和能谱（SEM-EDS）、水接触角、动态力学和 pH 值分析对所得支架进行表征。采用人乳牙牙髓干细胞（SHED）和大鼠实验研究支架的生物相容性。
SEM-EDS 证实成功制备了三维 PLGA-生物活性玻璃支架。结果表明，PLGA 中添加 10%的生物活性玻璃具有良好的性能，包括增加孔径、亲水性和机械性能。含有生物活性玻璃的支架的生长培养基 pH 值升高。所有支架均具有生物相容性，与其他支架相比，10%生物活性玻璃复合支架更有利于 SHED 的附着、生长和增殖。此外，它还增强了 SHED 在体外和体内的成骨分化。
成功建立了基于盐浸法的 PLGA-生物活性玻璃复合支架。PLGA 中添加 10%的生物活性玻璃具有足够的物理性能和生物活性，可考虑将其作为骨组织工程应用的复合材料。
