Key Laboratory of Chemical Biology & Traditional Chinese Medicine Research (Ministry of Education, China), College of Chemistry and Chemical Engineering, Hunan Normal University , Changsha 410081, People's Republic of China.
National & Local Joint Engineering Laboratory for New Petro-chemical Materials and Fine Utilization of Resources, College of Chemistry and Chemical Engineering, Hunan Normal University , Changsha 410081, People's Republic of China.
ACS Appl Mater Interfaces. 2017 Feb 8;9(5):4890-4897. doi: 10.1021/acsami.6b15032. Epub 2017 Jan 25.
In this study, bamboo fiber was first designed to incorporate into nano-hydroxyapatite/poly(lactic-co-glycolic) to obtain a new composite scaffold of bamboo fiber/nano-hydroxyapatite/poly(lactic-co- glycolic) (BF/n-HA/PLGA) by freeze-drying method. The effect of their components and some factors consisting of different freeze temperatures, concentrations, and pore-forming agents on the porous morphology, porosity, and compressive properties of the scaffold were investigated by scanning electron microscope, modified liquid displacement method, and electromechanical universal testing machine. The results indicated that the 5% BF/30% n-HA/PLGA composite scaffold, prepared with 5% (w/v) high concentration and frozen at -20 °C without pore-forming agent, had the best ideal porous structure and porosity as well as compressive properties, which far exceed those of n-HA/PLGA composite scaffold. In addition, the in vitro simulated body fluids soaking and cell culture experiment showed the addition of BF into the scaffold accelerated the BF/n-HA/PLGA composite scaffolds degradation and exhibited good cytocompatibility, including attachment and proliferation. All the results of the study show that BF has improved the properties of n-HA/PLGA composite scaffolds and BF/n-HA/PLGA might have a great potential for bone tissue engineering scaffold.
在这项研究中，首次设计将竹纤维纳入纳米羟基磷灰石/聚（乳酸-共-乙醇酸）中，通过冷冻干燥法获得一种新的竹纤维/纳米羟基磷灰石/聚（乳酸-共-乙醇酸）（BF/n-HA/PLGA）复合支架。通过扫描电子显微镜、改良的液体置换法和机电万能试验机研究了它们的成分以及由不同冷冻温度、浓度和造孔剂组成的一些因素对支架多孔形态、孔隙率和压缩性能的影响。结果表明，以 5%（w/v）高浓度和无造孔剂在-20°C 下冷冻制备的 5% BF/30% n-HA/PLGA 复合支架具有最佳的理想多孔结构、孔隙率和压缩性能，远远超过 n-HA/PLGA 复合支架。此外，体外模拟体液浸泡和细胞培养实验表明，BF 的加入加速了 BF/n-HA/PLGA 复合支架的降解，并表现出良好的细胞相容性，包括黏附和增殖。研究的所有结果表明，BF 改善了 n-HA/PLGA 复合支架的性能，BF/n-HA/PLGA 可能具有巨大的骨组织工程支架潜力。
