Department of Urology, Minimally Invasive Surgery Center, Guangdong Key Laboratory of Urology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510230, China.
Division of Urology, Department of Science, Intervention and Technology, Karolinska Institute, Stockholm, Sweden.
Urolithiasis. 2021 Oct;49(5):407-414. doi: 10.1007/s00240-021-01243-9. Epub 2021 Jan 17.
Previous clinical studies have shown that Escherichia coli (E. coli) predominated in urine and stone culture from calcium oxalate (CaOx) stone disease. The characteristic and relationship between E. coli isolated from urine cultures (EUC) and stone cultures (ESC) are compared. 83 E. coli (33 EUC and 50 ESC, respectively) from 66 CaOx stone patients were recruited in the study. E. coli in urine and stones from those patients were assessed by antimicrobial susceptibility test, genotyping and phylogenetic grouping. Furthermore, whole genome sequencing and comparative genomic analysis in paired ESC and EUC isolated strains from eight patients were carried out. The E. coli strains from ESC and EUC were not only multidrug resistant (MDR), but also had the similar pattern of resistant genes. The dominant phylogenetic group was B2, which was found in 54.0% of the ESC samples and 69.7% of the EUC samples, respectively. The virulence genes of E. coli, which isolated from stones and urine in the same patients, were highly homologous and largely consistent. Meanwhile, these E. coli strains were located in the same clade originated from a common ancestor. ESC and EUC isolated from patients with CaOx stones had a high prevalence of phylogenetic groups B2. Bacterial strains isolated from urine and stones in the same patient had consistent antimicrobial susceptibility profiles, genotyping, phylogenetic groups, virulence and resistance genes, also with high sequence co-linearity and close relationships.
先前的临床研究表明，大肠埃希菌（Escherichia coli，E. coli）在草酸钙（CaOx）结石病患者的尿液和结石培养物中占优势。本研究比较了来自尿液培养物（EUC）和结石培养物（ESC）的分离大肠埃希菌的特征和关系。本研究纳入了 66 例 CaOx 结石患者的 83 株大肠埃希菌（分别为 33 株 EUC 和 50 株 ESC）。对这些患者的尿液和结石中的大肠埃希菌进行了药敏试验、基因分型和系统发育群分析。此外，对 8 例患者的 ESC 和 EUC 分离株进行了全基因组测序和比较基因组分析。ESC 和 EUC 分离株不仅具有多重耐药性（MDR），而且耐药基因模式相似。优势的系统发育群为 B2，分别在 54.0%的 ESC 样本和 69.7%的 EUC 样本中发现。来自同一患者的结石和尿液中分离的大肠埃希菌的毒力基因高度同源，且基本一致。同时，这些大肠埃希菌菌株位于同一进化枝，起源于共同的祖先。来源于 CaOx 结石患者的 ESC 和 EUC 分离株中 B2 型大肠埃希菌的流行率较高。同一患者的尿液和结石中分离的细菌株具有一致的药敏谱、基因分型、系统发育群、毒力和耐药基因，也具有较高的序列一致性和密切的亲缘关系。
