Nesnow S, Easterling R E, Ellis S, Watts R, Ross J
Carcinogenesis and Metabolism Branch, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711.
Cancer Lett. 1988 Feb;39(1):19-27. doi: 10.1016/0304-3835(88)90036-5.
The metabolites of benz[j]aceanthrylene (B[j]A) produced by incubation with liver S9 proteins from rats induced with Aroclor-1254 and phenobarbital have been identified as: trans-B[j]A-1,2-dihydrodiol, B[j]A-9,10-dihydrodiol, B[j]A-11,12-dihydrodiol, and 10-hydroxy-B[j]A. The major metabolite formed (58-60%) by both induced S9 preparations was trans-B[j]A-1,2-dihydrodiol, the cyclopenta-ring dihydrodiol while oxidation at the k-region or the proximal-bay region was minor. There were no statistical differences in individual or total B[j]A metabolite rates between the 2 induced S9 preparations. B[l]A was metabolized by Aroclor-1254 and phenobarbital induced rat liver S9 preparations to trans-B[l]A-1,2-dihydrodiol, B[l]A-7,8-dihydrodiol, and B[l]A-4,5-dihydrodiol. The major B[l]A metabolite formed (28-40%) by both induced S9 preparations was B[l]A-7,8-dihydrodiol, the k-region dihydrodiol. Cyclopenta-ring oxidation to trans-B[l]A-1,2-dihydrodiol was approximately 50% of that observed for k-region oxidation. Both induced S9s produced similar rates of B[l]A metabolites except for B[l]A-7,8-dihydrodiol formation which was higher for Aroclor-1254-induced S9.
将苯并[j]刺蒽（B[j]A）与用多氯联苯混合物1254（Aroclor - 1254）和苯巴比妥诱导的大鼠肝脏S9蛋白一起孵育产生的代谢产物已被鉴定为：反式-B[j]A - 1,2 - 二氢二醇、B[j]A - 9,10 - 二氢二醇、B[j]A - 11,12 - 二氢二醇和10 - 羟基 - B[j]A。两种诱导的S9制剂形成的主要代谢产物（58 - 60%）是反式-B[j]A - 1,2 - 二氢二醇，即环戊环二氢二醇，而在k区域或近端湾区的氧化作用较小。两种诱导的S9制剂之间的单个或总B[j]A代谢产物速率没有统计学差异。苯并[l]A（B[l]A）被Aroclor - 1254和苯巴比妥诱导的大鼠肝脏S9制剂代谢为反式-B[l]A - 1,2 - 二氢二醇、B[l]A - 7,8 - 二氢二醇和B[l]A - 4,5 - 二氢二醇。两种诱导的S9制剂形成的主要B[l]A代谢产物（28 - 40%）是B[l]A - 7,8 - 二氢二醇，即k区域二氢二醇。环戊环氧化为反式-B[l]A - 1,2 - 二氢二醇的量约为k区域氧化量的50%。除了B[l]A - 7,8 - 二氢二醇的形成，Aroclor - 1254诱导的S9制剂中该产物的形成量更高外，两种诱导的S9制剂产生的B[l]A代谢产物速率相似。
