克隆性 B 细胞上 CD148 和 CD180 的相对表达水平及 CD148/CD180 中荧光强度比值有助于特征化成熟 B 细胞淋巴肿瘤浸润血液和骨髓 - 来自单中心初步研究的结果。
The relative expression levels of CD148 and CD180 on clonal B cells and CD148/CD180 median fluorescence intensity ratios are useful in the characterization of mature B cell lymphoid neoplasms infiltrating blood and bone marrow - Results from a single centre pilot study.
机构信息
Department of Hematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
Department of Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
出版信息
Int J Lab Hematol. 2021 Oct;43(5):1123-1131. doi: 10.1111/ijlh.13467. Epub 2021 Jan 17.
INTRODUCTION
The categorization of mature B cell neoplasms (MBN) infiltrating blood and bone marrow are met with difficulties. The inclusion of CD148 and CD180 in the routine flow cytometry/FCM panels has been suggested to refine the diagnosis. We studied the discriminatory ability of CD148 and CD180 median fluorescence intensity(MFI), CD148/CD180 ratio and their expression relative to T cells (CD148 , CD180 ), neutrophils (CD148 , CD180 ) and normal B cells (CD148 , CD180 ) in the differentiation of mature B cell neoplasms (MBN) especially non-chronic lymphocytic leukaemia (CLL).
METHODOLOGY
The flow cytometric (FCM) expression of CD148 and CD180 was studied prospectively in 102 patients (non-CLL; n = 72); diagnosed by a comprehensive panel of immunophenotypic and cytogenetic studies. The MFI and ratios were statistically compared across MBNs by Mann-Whitney U test. Cut-off values, sensitivity and specificity were calculated for significant parameters by receiver operator characteristic curve.
RESULTS
CD180MFI > 4.35 showed 100% sensitivity and 90.9% specificity for a diagnosis of marginal zone lymphoma (MZL) while, CD148/180 > 5.15 was 100% specific and 81.8% sensitive for lymphoplasmacytic lymphoma. CD148 (>4.3; 100% specificity, 83.4% sensitivity) and CD148 (>1.1; 100% sensitivity, 90% sensitivity) were useful for differentiating blastoid-mantle cell lymphoma/MCL from diffuse large B cell lymphoma; while CD148MFI (≥20.25), CD148 (>3.35) and CD148 (>0.95) showed >90% specificity and sensitivity for distinguishing MCL from CLL. Pairwise analysis also showed a good discriminant function of various parameters for distinguishing SMZL from other MBNs like FL, MCL as well as CLL.
CONCLUSIONS
The current study shows an excellent utility of CD148MFI, CD180MFI, their ratio and relative expression levels in the subcategorization of immunophenotypically related MBNs.
简介
成熟 B 细胞肿瘤（MBN）浸润血液和骨髓的分类存在困难。在常规流式细胞术/FCM 面板中加入 CD148 和 CD180 被认为可以改善诊断。我们研究了 CD148 和 CD180 中值荧光强度（MFI）、CD148/CD180 比值及其与 T 细胞（CD148、CD180）、中性粒细胞（CD148、CD180）和正常 B 细胞（CD148、CD180）表达的相对差异在成熟 B 细胞肿瘤（MBN），特别是非慢性淋巴细胞白血病（CLL）的鉴别诊断中的作用。
方法
前瞻性研究了 102 例（非 CLL；n=72）患者的 CD148 和 CD180 的流式细胞术（FCM）表达，这些患者通过免疫表型和细胞遗传学综合分析进行诊断。采用 Mann-Whitney U 检验对 MBN 之间的 MFI 和比值进行统计学比较。通过受试者工作特征曲线计算有意义参数的截断值、灵敏度和特异性。
结果
CD180MFI＞4.35 对边缘区淋巴瘤（MZL）的诊断具有 100%的灵敏度和 90.9%的特异性，而 CD148/180＞5.15 对淋巴浆细胞淋巴瘤具有 100%的特异性和 81.8%的灵敏度。CD148（＞4.3；100%特异性，83.4%灵敏度）和 CD148（＞1.1；100%灵敏度，90%灵敏度）有助于区分母细胞样-套细胞淋巴瘤/MCL 与弥漫性大 B 细胞淋巴瘤；而 CD148MFI（≥20.25）、CD148（＞3.35）和 CD148（＞0.95）对 MCL 与 CLL 的鉴别诊断具有＞90%的特异性和灵敏度。两两分析还表明，各种参数对区分 SMZL 与其他 MBN （如 FL、MCL 以及 CLL）具有良好的判别功能。
结论
本研究表明 CD148MFI、CD180MFI、它们的比值和相对表达水平在免疫表型相关 MBN 的细分中具有极好的应用价值。
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