Vector and Waterborne Pathogen Research Group, College of Science, Health, Engineering and Education, Murdoch University, Perth, WA, Australia.
School of Public Health, The University of Queensland, Herston, Qld, Australia.
Zoonoses Public Health. 2021 Jun;68(4):358-360. doi: 10.1111/zph.12806. Epub 2021 Jan 17.
In the present study, a 37-year-old immunosuppressed female in Western Australia (WA) was identified as positive for Cryptosporidium by microscopy and treated with nitazoxanide. Molecular analyses at the 18S ribosomal RNA (18S) and 60 kDa glycoprotein (gp60) loci identified C. fayeri subtype IVgA10G1T1R1, which had previously been identified in western grey kangaroos (Macropus fuliginosus) in WA. Next generation sequencing (NGS) of the gp60 locus confirmed the absence of mixed infections with other Cryptosporidium species. This is only the second report of C. fayeri in a human host highlighting the zoonotic potential of this wildlife-associated species. Routine diagnosis using molecular methods in laboratories is required to better understand the diversity and epidemiology of Cryptosporidium parasite.
在本研究中，西澳大利亚州（WA）的一名 37 岁免疫抑制女性通过显微镜检查被鉴定为隐孢子虫阳性，并接受硝唑尼特治疗。18S 核糖体 RNA（18S）和 60 kDa 糖蛋白（gp60）基因座的分子分析鉴定出 C. fayeri 亚型 IVgA10G1T1R1，此前在 WA 的西部灰袋鼠（Macropus fuliginosus）中也发现过这种亚型。gp60 基因座的下一代测序（NGS）证实不存在与其他隐孢子虫物种的混合感染。这是人类宿主中第二次报告 C. fayeri，突显了这种与野生动物相关的物种的人畜共患潜力。实验室中使用分子方法进行常规诊断，有助于更好地了解隐孢子虫寄生虫的多样性和流行病学。
