Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Vet Parasitol. 2011 Jun 30;179(1-3):22-7. doi: 10.1016/j.vetpar.2011.02.011. Epub 2011 Feb 23.
A total of 763 faecal samples were collected from western grey kangaroos (Macropus fuliginosus) in Western Australia and screened for the presence of Cryptosporidium by PCR at the 18S ribosomal RNA (rRNA) locus. Samples that were positive at the 18S locus were also amplified at the actin locus. The overall prevalence was 9.3% (71/763). At the 18S rRNA locus, sequences were obtained for 28 of the 71 positives. Sequence analysis identified four species; Cryptosporidium fayeri in seven isolates, Cryptosporidium marcopodum in four isolates, Cryptosporidium xiaoi in six isolates and a novel genotype (kangaroo genotype I) in eleven isolates. Analysis at the actin locus confirmed the genetic distinctness of the novel genotype. The results of the present study indicate that in addition to C. fayeri and C. marcopodum, kangaroos may be capable of being infected with a wider range of Cryptosporidium species and genotypes including livestock species such as C. xiaoi. The novel genotype identified in the kangaroos most likely represents a cryptic species that requires further analyses to confirm its species status.
从西澳大利亚的西部灰袋鼠（Macropus fuliginosus）中收集了总共 763 份粪便样本，并通过聚合酶链反应（PCR）在 18S 核糖体 RNA（rRNA）基因座上筛选是否存在隐孢子虫。在 18S 基因座上呈阳性的样本也在肌动蛋白基因座上进行了扩增。总体流行率为 9.3%（71/763）。在 18S rRNA 基因座上，从 71 个阳性样本中获得了 28 个序列。序列分析确定了四个种；在七个分离株中发现了隐孢子虫 fayeri，在四个分离株中发现了隐孢子虫 marcopodum，在六个分离株中发现了隐孢子虫 xiaoi，在十一个分离株中发现了一种新型基因型（袋鼠基因型 I）。肌动蛋白基因座的分析证实了新型基因型的遗传独特性。本研究的结果表明，除了 C. fayeri 和 C. marcopodum 之外，袋鼠可能还能够感染更广泛的隐孢子虫种和基因型，包括牛科动物等家畜种的隐孢子虫 xiaoi。在袋鼠中鉴定出的新型基因型很可能代表一种隐种，需要进一步分析来确认其种的地位。
