Bouzyk E, Rosiek O
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Dorodna, Poland.
Cancer Lett. 1988 Feb;39(1):93-9. doi: 10.1016/0304-3835(88)90044-4.
Treatment of mouse lymphoma L5178Y cells with spermine in Fischer's medium containing bovine serum lead to formation of chromatid aberrations. The cause of this effect seems to be that bovine serum contains spermine oxidase which catalyzes oxidation of spermine to unstable aldehyde derivatives (oxidized spermine) and hydrogen peroxide. Fraction of chromatid aberrations induced by oxidized spermine was estimated in experiments in which the cells were treated with spermine or a stoichiometric amount of hydrogen peroxide in the presence and in the absence of catalase. Cytotoxicity of oxidized spermine was evaluated by clonogenic assay.
在含有牛血清的费氏培养基中用精胺处理小鼠淋巴瘤L5178Y细胞会导致染色单体畸变的形成。这种效应的原因似乎是牛血清中含有精胺氧化酶，它催化精胺氧化为不稳定的醛衍生物（氧化精胺）和过氧化氢。在细胞分别在有和没有过氧化氢酶的情况下用精胺或化学计量的过氧化氢处理的实验中，估计了氧化精胺诱导的染色单体畸变率。通过克隆形成试验评估氧化精胺的细胞毒性。
