Gou Min, Huang Yi-Zhou, Hu Jun-Gen, Jiang Yan-Lin, Zhang Xiu-Zhen, Su Nai-Chuan, Lei Yi, Zhang Hai, Wang Hang, Xie Hui-Qi
State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of prosthodontics, West China Hospital of Stomatology, Sichuan University, No.14, 3rd Section, Ren Min Nan Rd., Chengdu 610041, China.
Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University and Collaborative Innovation Center of Biotherapy, No.1, Keyuan 4th Rd., Chengdu 610041, China.
ACS Biomater Sci Eng. 2019 Oct 14;5(10):5024-5035. doi: 10.1021/acsbiomaterials.9b00920. Epub 2019 Sep 27.
Collagen membranes are widely used in guided bone regeneration (GBR) because of their good biocompatibility and low immunogenicity. As a bioderived collagen membrane, small intestinal submucosa (SIS) has good regenerative potential for soft tissue repair, but it lacks sufficient mechanical properties for GBR application unless properly modifided. Epigallocatechin-3-gallate (EGCG) is a natural cross-linking agent featuring osteoinductive activity. In this study, we modified SIS by EGCG cross-linking, and such modified materials were characterized both and . The results showed that EGCG cross-linking significantly improved the mechanical properties and hydrophilicity of SIS while maintaing good cytocompatibility. Compared to SIS, EGCG-cross-linked SIS (E-SIS) enhanced the adhesion of fibroblasts and preosteoblasts and promoted the osteogenic differentiation of MC3T3-E1 cells cultured on the materials. In a rat cranial defect model, E-SIS material showed better occlusion effect than SIS material. Most importantly, E-SIS material accelerated bone regeneration more than SIS material and even a commercially available GBR membrane. Taken together, we conclude that E-SIS is a promising material as a GBR membrane.
胶原膜因其良好的生物相容性和低免疫原性而被广泛应用于引导骨再生（GBR）。作为一种生物衍生的胶原膜，小肠黏膜下层（SIS）在软组织修复方面具有良好的再生潜力，但除非经过适当改性，否则它缺乏足够的力学性能用于GBR应用。表没食子儿茶素-3-没食子酸酯（EGCG）是一种具有骨诱导活性的天然交联剂。在本研究中，我们通过EGCG交联对SIS进行改性，并对这种改性材料进行了 和 表征。结果表明，EGCG交联显著提高了SIS的力学性能和亲水性，同时保持了良好的细胞相容性。与SIS相比，EGCG交联的SIS（E-SIS）增强了成纤维细胞和前成骨细胞的黏附，并促进了在材料上培养的MC3T3-E1细胞的成骨分化。在大鼠颅骨缺损模型中，E-SIS材料比SIS材料显示出更好的封闭效果。最重要的是，E-SIS材料比SIS材料甚至一种市售的GBR膜更能加速骨再生。综上所述，我们得出结论，E-SIS作为一种GBR膜是一种有前途的材料。
