Improved of growth and phytostabilization potential of lead (Pb) in L. under the effect of IAA and GA alone and in combination with EDTA by altering biochemical attributes of stressed plants.

This study evaluated the effect of EDTA, IAA and GA alone and in combination in improving plant growth, Pb accumulation, and management of effective mechanisms associated with alleviation of Pb-induced adverse effect in L. grown on industrial area in Alexandria. In this regards, 10-week-old plants were subjected to IAA and GA as a foliar spray and EDTA was supplied in two doses 50 and 200mgkg soil. EDTA significantly reduced the plant growth and dry biomass, whereas GA and IAA foliar spray increased growth significantly when compared with control (uncontaminated soil). In combined treatments of EDTA + GA +IAA, the biomass was restored, which shows that GA and IAA did compensate the negative effect of EDTA on plant growth and increased the Pb uptake significantly into roots. There were high GSH contents parallel with the increase of glutathione-S-transferase activity and induction of the antioxidant enzymes (SOD, CAT, APX, GR) as well as oxidized glutathione and ascorbic acid contents in leaves and roots when compared to control plants. This study suggests that is promising species for decontamination of Pb -contaminated soil and the application of EDTA together with IAA and GA could be a useful strategy for enhancing the phytostabilization capability of L. to eliminate Pb from contaminated soils. : The objective of this paper is to investigate the physiological performance of as a new native for phytoremediation by phytostabilization mechanism of Pb after treatment with EDTA and phytohormones. Chemical additives of EDTA and GA as well as IAA are promising alternatives to provide added benefits due to their individual credentials in improving the overall phytostabilization effectiveness and better immobilization efficiency in treating Pb contaminated soils by altering biochemical attributes of stressed plants. Currently these additives are not employed widely in large-scale field implementations, so field applications of these additives using are essential for Phytoremediation of Pb-contaminated soils.