Denny P A, Pimprapaiporn W, Kim M S, Denny P C
Department of Basic Sciences, School of Dentistry, University of Southern California, Los Angeles 90089.
Cell Tissue Res. 1988 Feb;251(2):381-6. doi: 10.1007/BF00215847.
In this study, antiserum to acinar cell-specific mucin was utilized to determine whether mucin could be detected in the mouse submandibular gland prior to cytodifferentiation of acinar cells. Results from radioimmunoassay indicated that mucin occurs in submandibular glands from newborn mice, i.e., before the appearance of mature acinar cells. Additionally, mucin quantitated in various stages of development was found to be antigenically identical to adult mucin. After sections of glands were treated with immunohistochemical reagents, we observed that the mature acinar cell-specific mucin was present in secretory terminal-tubule cells and in proacinar cells of newborn animals. The present findings suggest that in young animals, the proacinar cells are an immediate precursor of acinar cells and that the secretory terminal-tubule cells may represent an earlier stage in development of acinar cells. In adult female glands, mucin was also detected in the granular intercalating-duct cells. This latter observation is consistent with the hypothesis that these cells are an intermediate in the acinar cell replacement process.
在本研究中，利用腺泡细胞特异性黏蛋白抗血清来确定在腺泡细胞进行细胞分化之前，是否能在小鼠下颌下腺中检测到黏蛋白。放射免疫分析结果表明，新生小鼠的下颌下腺中存在黏蛋白，即在成熟腺泡细胞出现之前。此外，发现在发育各个阶段定量的黏蛋白在抗原性上与成年黏蛋白相同。在用免疫组化试剂处理腺体切片后，我们观察到成熟腺泡细胞特异性黏蛋白存在于新生动物的分泌终末小管细胞和前腺泡细胞中。目前的研究结果表明，在幼龄动物中，前腺泡细胞是腺泡细胞的直接前体，并且分泌终末小管细胞可能代表腺泡细胞发育的早期阶段。在成年雌性腺体中，在颗粒性闰管细胞中也检测到了黏蛋白。后一观察结果与这些细胞是腺泡细胞替代过程中的中间体这一假设一致。
