National Institute of Health Sciences, Kanagawa, Japan.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2021 Mar;38(3):409-417. doi: 10.1080/19440049.2020.1869326. Epub 2021 Jan 16.
Progesterone (P4) is contained naturally in animal tissue, and it is also used as a veterinary drug in cattle for treatment purposes. To assess the risk from P4 residues in beef derived from treated cattle, it is essential to quantify the P4 contained naturally in cattle tissue (endogenous P4). Therefore, we performed a method validation for the quantification of endogenous P4 (method quantification limit = 0.06 ng g) by using isotope-labelled P4s, and investigated the P4 contents in Japanese beef (= 112; 0.07 to 121 ng g). The P4 contents in cattle muscle ranged from 0.07 to 54.3 ng g in males, and from 0.27 to 121 ng g in females. Our investigation also indicated that the developed method using both - and deuterium-labelled P4 standards could be used to certify the recovery of P4 from cattle muscle containing various amounts of intramuscular fat, and enabled the determination of the P4 content in all Japanese beef samples that exceeded the method quantification limit.
孕酮（P4）天然存在于动物组织中，也被用作牛的兽医药物进行治疗。为了评估来源于用 P4 处理过的牛的牛肉中 P4 残留的风险，定量测定牛组织中天然存在的 P4（内源性 P4）是至关重要的。因此，我们使用同位素标记的 P4 对 P4 （方法定量限=0.06ng g）的定量进行了方法验证，并对日本牛肉中的 P4 含量（=112；0.07 至 121ng g）进行了调查。雄性牛肌肉中的 P4 含量范围为 0.07 至 54.3ng g，雌性牛肌肉中的 P4 含量范围为 0.27 至 121ng g。我们的研究还表明，使用 - 和氘标记的 P4 标准品开发的方法可用于证明从含有不同肌内脂肪量的牛肌肉中回收 P4 的回收率，并且能够测定所有超出方法定量限的日本牛肉样品中的 P4 含量。
