Fertility Medical Group, Av. Brigadeiro Luis Antonio, 4545, São Paulo, SP01401-002, Brazil.
Sapientiae Institute - Centro de Estudos e Pesquisa em Reprodução Humana Assistida, Rua Vieira Maciel, 62 São Paulo, SP04503-040, Brazil.
Zygote. 2021 Jun;29(3):234-238. doi: 10.1017/S0967199420000842. Epub 2021 Jan 18.
The objective of this study was to investigate any effect of cryopreservation of donated eggs on laboratorial and clinical outcomes of intracytoplasmic sperm injection (ICSI) cycles. This retrospective cohort study included 320 oocyte recipients undergoing 307 vitrified and 119 fresh oocyte recipient ICSI cycles, participating in an egg-sharing donation programme, from 2015 to 2018, in a private university-affiliated in vitro fertilization (IVF) centre. A review of donor and recipient ICSI cycles was charted. A general mixed models fit by restricted maximum likelihood, followed by Bonferroni post hoc test was used to compare the means between fresh and warm oocyte donation groups and investigate the effect of cryopreservation on recipient ICSI outcome. The main outcome measure was blastocyst development rates. Fertilization rate, high-quality embryo rates on days 2 and 3, normal cleavage speed rates on days 2 and 3, and blastocyst development rate were significantly higher for the fresh oocyte donation cycles compared with warmed oocyte donation cycles. In the egg-sharing donation programme, fertilization and embryo developmental competence were reduced when vitrified oocytes from infertile couples were used for ICSI compared with fresh oocytes.
本研究旨在探究捐赠卵子冷冻保存对卵胞浆内单精子注射（ICSI）周期实验室和临床结局的影响。本回顾性队列研究纳入了 2015 年至 2018 年期间在一家私立大学附属体外受精（IVF）中心参与卵子共享捐赠计划的 320 名接受 307 个冷冻和 119 个新鲜卵子接受 ICSI 周期的卵母细胞受体。对供体和受体 ICSI 周期进行了图表回顾。采用受限最大似然法的广义混合模型拟合，随后进行 Bonferroni 事后检验，比较新鲜和 warmed 卵母细胞捐赠组之间的均值，并研究冷冻保存对受体 ICSI 结局的影响。主要结局指标是囊胚发育率。与 warmed 卵母细胞捐赠周期相比，新鲜卵母细胞捐赠周期的受精率、第 2 天和第 3 天的优质胚胎率、第 2 天和第 3 天的正常卵裂速度率以及囊胚发育率均显著更高。在卵子共享捐赠计划中，与新鲜卵母细胞相比，使用来自不孕夫妇的冷冻卵母细胞进行 ICSI 会降低受精和胚胎发育能力。
