Shinkai Y, Ishikawa H, Hattori M, Okumura K
Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan.
Eur J Immunol. 1988 Jan;18(1):29-33. doi: 10.1002/eji.1830180106.
Pore-forming protein (perforin, PFP) was isolated from a mouse large granular lymphocyte (LGL) [natural killer (NK-like)] cell line. Purified PFP lysed a variety of mouse tumor cell lines and helper T lymphocyte cell lines. However, LGL and cytotoxic T lymphocyte cell lines were resistant to PFP-mediated cell lysis. The presence of hemolytic activity in the granule was examined in these resistant cell lines. Four out of five of these resistant cell lines had hemolytically active granules. We determined whether NK cells freshly isolated from BALB/c nude mouse spleens were resistant to PFP-mediated cytolysis. Nylon column-passed spleen cells with an enriched content of NK cells exhibited more resistance than whole spleen cells. Moreover, when spleen cells were treated with PFP the remaining live cells showed enriched NK activity suggesting that normal peripheral cells with NK activity are resistant to PFP. These results indicate that cytolytic cells containing PFP have developed defense mechanisms to inhibit PEP-mediated cell lysis.
成孔蛋白（穿孔素，PFP）从小鼠大颗粒淋巴细胞（LGL）[自然杀伤（NK样）]细胞系中分离得到。纯化的PFP可裂解多种小鼠肿瘤细胞系和辅助性T淋巴细胞系。然而，LGL和细胞毒性T淋巴细胞系对PFP介导的细胞裂解具有抗性。我们检测了这些抗性细胞系中颗粒内溶血活性的存在情况。在这五个抗性细胞系中，有四个具有溶血活性颗粒。我们确定了从BALB/c裸鼠脾脏中新鲜分离的NK细胞是否对PFP介导的细胞溶解具有抗性。经过尼龙柱处理、NK细胞含量富集的脾细胞比全脾细胞表现出更强的抗性。此外，当脾细胞用PFP处理时，剩余的活细胞显示出富集的NK活性，这表明具有NK活性的正常外周细胞对PFP具有抗性。这些结果表明，含有PFP的溶细胞性细胞已发展出防御机制来抑制PEP介导的细胞裂解。
