Six additional mitochondrial genomes for North American nightsnakes (Dipsadidae: ) and a novel gene feature for advanced snakes.

The North American nightsnakes in the genus is composed of nine named and at least two unnamed species. Here, we provide the first mt-genome of , an additional mt-genome for sp. nov. 1, and four additional mt-genomes from the widespread . These mtDNA genomes were sequenced using both Illumina and Ion Torrent sequencing technologies. The resulting genomes contained the expected 13 protein coding genes, 22 tRNA genes, 2 rRNA genes, and 2 control regions typical of colubroid snakes. Two of the . samples had partial tRNA genes upstream of CR2 which has not been previously documented in colubroid snakes. A maximum likelihood gene-tree based on these data combined with previously published sequence data recovers a well-supported phylogeny and is in concordance with previous estimates of evolutionary relationships in this group.