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下一代测序技术实现了和平斗鱼(硬骨鱼纲:丝足鲈科)线粒体基因组的完整组装。

Next-generation sequencing yields complete mitochondrial genome assembly of peaceful betta fish, (Teleostei: Osphronemidae).

作者信息

Ahmad Syed Farhan, Laopichienpong Nararat, Singchat Worapong, Suntronpong Aorarat, Pongsanarm Tavun, Panthum Thitipong, Ariyaraphong Nattakan, Bulan Jakaphan, Pansrikaew Tanawat, Jangtarwan Kornsuang, Subpayakom Navapong, Dokkaew Sahabhop, Muangmai Narongrit, Duengkae Prateep, Srikulnath Kornsorn

机构信息

Department of Genetics, Faculty of Science, Laboratory of Animal Cytogenetics and Comparative Genomics (ACCG), Kasetsart University, Bangkok, Thailand.

Department of Forest Biology, Faculty of Forestry, Special Research Unit for Wildlife Genomics (SRUWG), Kasetsart University, Bangkok, Thailand.

出版信息

Mitochondrial DNA B Resour. 2020 Dec 24;5(4):3856-3858. doi: 10.1080/23802359.2020.1841582.

Abstract

The complete mitochondrial genome (mitogenome) of the peaceful betta () was obtained using next-generation sequencing. The sample of was collected from its native habitat in Southern Thailand. The mitogenome sequence was 16,897 bp in length, containing 37 genes with identical order to most teleost mitogenomes. Overall nucleotide base composition of the complete mitogenome was determined as AT bias. Phylogenetic analysis of showed a closer relationship with bubble-nesting fighting fish. This annotated mitogenome reference can be utilized as a bioresource for phylogenetic studies to support betta conservation programs.

摘要

通过下一代测序获得了和平斗鱼()的完整线粒体基因组(线粒体基因组)。该斗鱼样本采自泰国南部的原生栖息地。线粒体基因组序列长度为16,897 bp,包含37个基因,其排列顺序与大多数硬骨鱼线粒体基因组相同。完整线粒体基因组的总体核苷酸碱基组成表现出A/T偏好。对和平斗鱼的系统发育分析表明,它与泡巢斗鱼的关系更为密切。这个注释的线粒体基因组参考可作为一种生物资源用于系统发育研究,以支持斗鱼保护计划。

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