Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina.
Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, Chacabuco 461, San Miguel de Tucumán, Tucumán, T4000ILI, Argentina.
Reprod Domest Anim. 2021 Apr;56(4):684-687. doi: 10.1111/rda.13896. Epub 2021 Feb 5.
In the present work, we established and characterized a 3D functional polarized primary bovine oviduct epithelial cells (BOECs) culture on free-floating type I collagen hydrogels (rafts) at an air-liquid interface (ALI). Intercellular junctions, ultrastructural cellular morphology and the expression of the OVGP1 closely recapitulated those of the in vivo epithelium lining. These morphological and physiological epithelial cell features were maintained under standard DMEM/F12 with 10% foetal bovine serum culture medium for at least 28 days of ALI culture. The versatility of the BOECs raft cultures should allow testing of toxicity compounds, in vitro evaluation of physiological or pathological oviductal states, and the study of epithelial-mesenchymal interactions that are critical for the maintenance of oviductal homeostasis.
在本工作中，我们在气液界面（ALI）上建立并表征了一种在游离型 I 型胶原水凝胶（筏）上的 3D 功能性极化原代牛输卵管上皮细胞（BOECs）培养。细胞间连接、超微结构细胞形态和 OVGP1 的表达与体内上皮衬里非常相似。这些形态学和生理学上皮细胞特征在至少 28 天的 ALI 培养中，使用含 10%胎牛血清的标准 DMEM/F12 培养基维持。BOECs 筏培养的多功能性应允许测试毒性化合物，体外评估生理或病理输卵管状态，并研究上皮-间充质相互作用，这对于维持输卵管内稳态至关重要。
