1Department of Genetics, Faculty of Medicine Veterinary and Zootechnics, Autonomous University of Nuevo León, Av. Francisco Villa s/n, Ex Hacienda el Canadá, 66050 Gral. Escobedo, NL, Mexico.
2Department of Histology, Faculty of Medicine, Autonomous University of Nuevo León, Mexico.
Acta Vet Hung. 2021 Jan 15;68(4):374-379. doi: 10.1556/004.2020.00045.
The Wilms' tumour gene (WT1) has previously been described as an oncogene in several neoplasms of humans, including melanoma, and its expression increases cancer cell proliferation. Recent reports associate the expression of the PPARβ/δ gene (peroxisome proliferator-activated receptor beta/delta) with the downregulation of WT1 in human melanoma and murine melanoma cell lines. The aim of this work was to analyse the expression of WT1 and its association with PPARβ/δ in samples of healthy and melanoma-affected skin of horses by immunohistochemistry. WT1 protein expression was detected in healthy skin, mainly in the epidermis, hair follicle, sebaceous gland and sweat gland, while no expression was observed in equine melanoma tissues. Moreover, it was observed that PPARβ/δ has a basal expression in healthy skin and that it is overexpressed in melanoma. These results were confirmed by a densitometric analysis, where a significant increase of the WT1-positive area was observed in healthy skin (128.66 ± 19.84 pixels 106) compared with that observed in melanoma (1.94 ± 0.04 pixels 106). On the other hand, a positive area with an expression of PPARβ/δ in healthy skin (214.94 ± 11.85 pixels 106) was significantly decreased compared to melanoma (624.86 ± 181.93 pixels 106). These data suggest that there could be a regulation between WT1 and PPARβ/δ in this disease in horses.
Wilms 瘤基因（WT1）先前已被描述为人类多种肿瘤的癌基因，包括黑色素瘤，其表达可增加癌细胞增殖。最近的报告将 PPARβ/δ 基因（过氧化物酶体增殖物激活受体β/δ）的表达与人类黑色素瘤和鼠黑色素瘤细胞系中 WT1 的下调相关联。本工作旨在通过免疫组织化学分析健康和受黑色素瘤影响的马皮肤样本中 WT1 的表达及其与 PPARβ/δ 的关联。WT1 蛋白在健康皮肤中表达，主要在表皮、毛囊、皮脂腺和汗腺中表达，而在马的黑色素瘤组织中未观察到表达。此外，观察到 PPARβ/δ 在健康皮肤中有基础表达，而在黑色素瘤中过度表达。通过密度分析证实了这些结果，其中在健康皮肤（128.66 ± 19.84 像素 106）中观察到 WT1 阳性区域的显著增加，与在黑色素瘤（1.94 ± 0.04 像素 106）中观察到的相比。另一方面，在健康皮肤中 PPARβ/δ 表达的阳性区域（214.94 ± 11.85 像素 106）与黑色素瘤（624.86 ± 181.93 像素 106）相比显著降低。这些数据表明，在马的这种疾病中，WT1 和 PPARβ/δ 之间可能存在调节关系。
