Programa de Pós-Graduação em Biologia Animal, UFRRJ, Seropédica, RJ, Brazil.
Departamento de Genética, Instituto de Ciências Biológicas e da Saúde, UFRRJ, Seropédica, RJ, Brazil.
Primates. 2021 Mar;62(2):395-406. doi: 10.1007/s10329-020-00880-9. Epub 2021 Jan 18.
The black-horned capuchin (Sapajus nigritus) is a neotropical primate with wide distribution from southeastern Brazil to northeastern Argentina. Although this species has been described with coat pattern variation, even with intrapopulational differences, and characterized as having the greatest genetic diversity among Sapajus species, there are still few studies on natural populations that contribute to the knowledge of this intraspecific variability. We examined individuals from an as yet unstudied population of Ilha da Marambaia, Rio de Janeiro (RJ) state, Brazil, compared with published data for S. nigritus. We sought to confirm the species through phenotypic and genetic characterization using C-banding and fluorescence in situ hybridization with #11qHe+/21WCP probes for chromosomal constitutive heterochromatin (He+) patterns, and cytochrome c oxidase I and II gene sequences for phylogenetic analysis. The coat presented two color patterns, varying from brown to blackish on the body, yellow to brown on the chest, and white to yellow on the face, besides the presence and shape of the tufts on the head, corresponding to S. nigritus. He+ was identified in pairs 4, 12, 13 and 17, and less consistently in pairs 6, 19 and 21, already described for this species. While most Sapajus species have a large He+ block, here pair 11 was identified without extracentromeric He+, the same as reported for S. nigritus from Argentina. Molecular analysis showed divergence of this population from other S. nigritus sequences, reinforcing a trend already demonstrated when samples from RJ are compared with the rest of the distribution, which may represent an evolutionary deviation.
黑角卷尾猴（Sapajus nigritus）是一种分布于从巴西东南部到阿根廷东北部的新热带灵长类动物。尽管该物种的皮毛图案存在变异，甚至存在种群内差异，并且被认为是 Sapajus 物种中遗传多样性最高的物种，但关于自然种群的研究仍然很少，这对该种内变异性的认识贡献有限。我们检查了来自巴西里约热内卢州马拉马亚伊岛（Ilha da Marambaia）一个尚未研究过的种群的个体，并与 Sapajus nigritus 的已发表数据进行了比较。我们试图通过表型和遗传特征来确认该物种，使用 C-带和荧光原位杂交技术，用#11qHe+/21WCP 探针检测染色体组成性异染色质（He+）模式，以及细胞色素 c 氧化酶 I 和 II 基因序列进行系统发育分析。该物种的皮毛呈现出两种颜色模式，身体上从棕色到黑色，胸部从黄色到棕色，面部从白色到黄色，除了头部的簇毛的存在和形状外，这与 Sapajus nigritus 相对应。He+在第 4、12、13 和 17 对中被识别出来，在第 6、19 和 21 对中则不太一致，这些已经在该物种中描述过。虽然大多数 Sapajus 物种都有一个大的 He+块，但这里的第 11 对没有额外的着丝粒 He+，这与来自阿根廷的 Sapajus nigritus 的报告相同。分子分析显示该种群与其他 Sapajus nigritus 序列的分化，这加强了 RJ 样本与分布其他地区的样本进行比较时已经显示出的趋势，这可能代表一种进化偏差。
