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[大肠杆菌K-12中的单链结合:异质后代形成的特征]

[Single-stranded conjugation in Escherichia coli K-12: characteristics of the formation of a heterogenous progeny].

作者信息

Goryshin I Iu, Lantsov V A

出版信息

Genetika. 1977;13(7):1246-51.

PMID:334630
Abstract

During a single-stranded conjugation donor DNA, being a single-stranded form, takes part in the process of recombination. That is why a heteroduplex DNA must be an intermediate product of the recombination. The heteroduplex can be partially corrected as it was supposed for genetic transformation. The division of such a corrected heteroduplex gives the heterogenous progeny of exoconjugants. And this "correctional" heterogeneity must possess two following properties: 1) the mixed progeny must consist of only two recombinational genotypes; 2) the heterogeneity must be marker-specific. The experimental support to both predictions was obtained by the method of clonal analysis of conjugational merozygotes.

摘要

在单链接合过程中,作为单链形式的供体DNA参与重组过程。这就是为什么异源双链DNA必定是重组的中间产物。异源双链可以像在遗传转化中所推测的那样进行部分校正。这种校正后的异源双链的分裂产生外接合子的异质后代。并且这种“校正性”异质性必须具备以下两个特性:1)混合后代必须仅由两种重组基因型组成;2)异质性必须是标记特异性的。通过对接合部分二倍体进行克隆分析的方法,获得了对这两个预测的实验支持。

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