Department of Immunology, School of Medicine, Iran University of Medical Science, Tehran, Iran.
Department of Immunology, School of Medicine, Iran University of Medical Science, Tehran, Iran AND Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2020 Aug 25;19(4):362-372. doi: 10.18502/ijaai.v19i4.4111.
Thymic stromal lymphopoietin (TSLP) is a cytokine similar to IL-7, which is released by airway epithelial cells in response to injury and inflammation. Current literature is contradictory about the association between different single nucleotide polymorphisms (SNPs) of the TSLP gene and asthma development in different countries. We aimed to evaluate the association between two common TSLP SNPs (rs2289276 and rs2289278) and the risk of asthma in the Iranian population. Genotyping of the TSLP gene was performed in 126 adult asthmatic patients and 300 controls; using the TaqMan genotyping assay. Moreover, total serum IgE level and eosinophil count were assessed. The results indicated that the TT genotype of rs2289276 was inversely associated with the risk of asthma (p=0.002). A similar inverse association was detected in subgroups of atopic (p=0.001) and non-atopic (p=0.005) asthma. Moreover, the TT genotype of this SNP was more prevalent in severe and late-onset categories of asthma. In subgroup analysis, a significant sex-specific association between rs2290276 and asthma was observed in women (p=0.004). The prevalence of rs2289276 was extremely low, which made it infeasible to perform any further analysis. Overall, our findings indicated that rs2290276 SNP of the TSLP gene has a protective phenotype against asthma development in the Iranian population.
胸腺基质淋巴细胞生成素 (TSLP) 是一种类似于白细胞介素-7 的细胞因子，它是气道上皮细胞在受到损伤和炎症刺激时释放的。目前的文献对于 TSLP 基因的不同单核苷酸多态性 (SNP) 与不同国家哮喘发展之间的关联存在争议。我们旨在评估两个常见的 TSLP SNP(rs2289276 和 rs2289278)与伊朗人群哮喘风险之间的关联。使用 TaqMan 基因分型检测法，对 126 例成年哮喘患者和 300 例对照者的 TSLP 基因进行了基因分型。此外，还评估了总血清 IgE 水平和嗜酸性粒细胞计数。结果表明，rs2289276 的 TT 基因型与哮喘风险呈负相关(p=0.002)。在特应性(p=0.001)和非特应性(p=0.005)哮喘亚组中也检测到类似的负相关。此外，该 SNP 的 TT 基因型在严重和迟发性哮喘类别中更为常见。在亚组分析中，rs2290276 与哮喘之间存在显著的性别特异性关联(p=0.004)。rs2289276 的患病率极低，因此无法进行进一步的分析。总体而言，我们的研究结果表明，TSLP 基因的 rs2290276 SNP 对伊朗人群哮喘的发生具有保护表型。
