Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing, People's Republic of China.
College of Plant Sciences and Technology, Huazhong Agricultural University, Wuhan, Hubei, 430070, People's Republic of China.
Environ Sci Pollut Res Int. 2021 Feb;28(8):9002-9019. doi: 10.1007/s11356-021-12419-6. Epub 2021 Jan 19.
Plant diseases significantly impact the global economy, and plant pathogenic microorganisms such as nematodes, viruses, bacteria, fungi, and viroids may be the etiology for most infectious diseases. In agriculture, the development of disease-free plants is an important strategy for the determination of the survival and productivity of plants in the field. This article reviews biosensor methods of disease detection that have been used effectively in other fields, and these methods could possibly transform the production methods of the agricultural industry. The precise identification of plant pathogens assists in the assessment of effective management steps for minimization of production loss. The new plant pathogen detection methods include evaluation of signs of disease, detection of cultured organisms, or direct examination of contaminated tissues through molecular and serological techniques. Laboratory-based approaches are costly and time-consuming and require specialized skills. The conclusions of this review also indicate that there is an urgent need for the establishment of a reliable, fast, accurate, responsive, and cost-effective testing method for the detection of field plants at early stages of growth. We also summarized new emerging biosensor technologies, including isothermal amplification, detection of nanomaterials, paper-based techniques, robotics, and lab-on-a-chip analytical devices. However, these constitute novelty in the research and development of approaches for the early diagnosis of pathogens in sustainable agriculture.
植物病害严重影响全球经济，而线虫、病毒、细菌、真菌和类病毒等植物病原微生物可能是大多数传染病的病因。在农业中，培育无病植物是确定植物在田间生存和生产力的重要策略。本文综述了已在其他领域有效应用的疾病检测生物传感器方法，这些方法可能会改变农业产业的生产方式。对植物病原体的精确识别有助于评估减少生产损失的有效管理措施。新的植物病原体检测方法包括评估疾病迹象、检测培养物或通过分子和血清学技术直接检查污染组织。基于实验室的方法成本高且耗时，并且需要专门的技能。本综述的结论还表明，迫切需要建立一种可靠、快速、准确、响应迅速且具有成本效益的检测方法，以在生长早期检测田间植物。我们还总结了新出现的生物传感器技术，包括等温扩增、纳米材料检测、基于纸张的技术、机器人技术和芯片实验室分析设备。然而，这些在可持续农业中对病原体进行早期诊断的方法的研究和开发中构成了新颖性。
