Šašić Zorić Ljiljana, Janjušević Ljiljana, Djisalov Mila, Knežić Teodora, Vunduk Jovana, Milenković Ivanka, Gadjanski Ivana
BioSense Institute, University of Novi Sad, Dr Zorana Đinđića 1, 21000 Novi Sad, Serbia.
Institute of General and Physical Chemistry, University of Belgrade, Studentski trg 12/V, 11080 Belgrade, Serbia.
Biology (Basel). 2023 Feb 14;12(2):299. doi: 10.3390/biology12020299.
Due to the evident aggressive nature of green mold and the consequently huge economic damage it causes for producers of edible mushrooms, there is an urgent need for prevention and infection control measures, which should be based on the early detection of various spp. as green mold causative agents. The most promising current diagnostic tools are based on molecular methods, although additional optimization for real-time, in-field detection is still required. In the first part of this review, we briefly discuss cultivation-based methods and continue with the secondary metabolite-based methods. Furthermore, we present an overview of the commonly used molecular methods for species/strain detection. Additionally, we also comment on the potential of genomic approaches for green mold detection. In the last part, we discuss fast screening molecular methods for the early detection of infestation with the potential for in-field, point-of-need (PON) application, focusing on isothermal amplification methods. Finally, current challenges and future perspectives in diagnostics are summarized in the conclusions.
由于绿霉菌具有明显的侵袭性，会给食用菌生产者造成巨大的经济损失，因此迫切需要采取预防和感染控制措施，这些措施应基于对作为绿霉菌病原体的各种物种的早期检测。目前最有前景的诊断工具基于分子方法，不过仍需要针对实时现场检测进行进一步优化。在本综述的第一部分，我们简要讨论基于培养的方法，接着介绍基于次生代谢产物的方法。此外，我们概述了用于物种/菌株检测的常用分子方法。此外，我们还评论了基因组方法在绿霉菌检测中的潜力。在最后一部分，我们讨论用于早期检测感染的快速筛选分子方法及其在现场即时检测（PON）中的应用潜力，重点关注等温扩增方法。最后，在结论中总结了绿霉菌诊断当前面临的挑战和未来展望。
