Institute of Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, China.
Department of Urology, Guangzhou Institute of Urology, Guangdong Key Laboratory of Urology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510230, China.
ACS Biomater Sci Eng. 2021 Feb 8;7(2):739-751. doi: 10.1021/acsbiomaterials.0c01318. Epub 2021 Jan 19.
An oxidative damage model of human proximal renal epithelial cells (HK-2) was established using oxalate damage. The repair effects of polysaccharide (APS) and selenized APS (Se-APS) on damaged HK-2 cells were investigated. Differences in the adhesion and endocytosis of HK-2 cells to calcium oxalate dihydrate crystals with a size of approximately 100 nm before and after APS and Se-APS repair were also explored. The results showed that after being repaired by APS and Se-APS, HK-2 cells exhibited increased cell viability, restored cell morphology, reduced reactive oxygen species level, increased mitochondrial membrane potential, reduced phosphatidylserine eversion, and osteopontin expression. Moreover, the amount of adherent crystals on the cell surface decreased, but the amount of endocytic crystals increased. At the same concentration, Se-APS exhibited better repair effects on the damaged HK-2 cells than APS. All these findings revealed that Se-APS may be a potential drug candidate for inhibiting the formation of kidney stones.
采用草酸盐损伤法建立人近端肾小管上皮细胞（HK-2）的氧化损伤模型，研究了多糖（APS）和硒化 APS（Se-APS）对受损 HK-2 细胞的修复作用。还探讨了 APS 和 Se-APS 修复前后 HK-2 细胞对大小约为 100nm 的二水草酸钙晶体的黏附和内吞作用的差异。结果表明，APS 和 Se-APS 修复后，HK-2 细胞活力增加，细胞形态恢复，活性氧水平降低，线粒体膜电位升高，磷脂酰丝氨酸外翻减少，骨桥蛋白表达降低。此外，细胞表面黏附晶体的量减少，而内吞晶体的量增加。在相同浓度下，Se-APS 对受损 HK-2 细胞的修复效果优于 APS。这些发现表明，Se-APS 可能是一种抑制肾结石形成的潜在药物候选物。
