Department of Reproductive Medicine and Genetics Center, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, P. R. China.
Department of Gynaecology and Obstetrics, The First Affiliated Hospital of GuangXi Medical University, Nanning, P. R. China.
Organogenesis. 2021 Apr 3;17(1-2):1-13. doi: 10.1080/15476278.2020.1860424. Epub 2021 Jan 19.
As a chaperone protein of progesterone receptor (PR), FK-506 Binding Protein 52 (FKBP52) can enhance the activity of PR, but the mechanism of FKBP52 affecting PR expression levels is difficult to clarify. Here, we report a novel in vitro model of ectopic endometrial stromal cells (ESCM) established through the primary culture method of endometrial stromal cells, which is used to study the details of relationship between FKBP52 abnormality and PR expression level in endometriosis (Ems). At the same time, the clinical study of the relationship between FKBP52 and PR expression levels in endometriosis patients was used to verify our conclusions. The results showed that the expression levels of PR-A mRNA and protein in endometriosis are positively correlated with FKBP52 and the abnormality of FKBP52 leads to the decrease of PR-B mRNA and protein expression. When FKBP52 was deleted or reduced, the expression levels of m RNA and protein of PR-A and PR-B have decreased leading to the proliferation of ectopic endometrium cells (ESC) and the occurrence of endometriosis, which is consistent with the expression levels of clinical endometriosis patients and fully confirms our conclusions and reliability of the model, and has great guiding significance for the research of Ems disease occurrence mechanism and clinical treatment.
作为孕激素受体（PR）的伴侣蛋白，FK-506 结合蛋白 52（FKBP52）可以增强 PR 的活性，但 FKBP52 影响 PR 表达水平的机制尚不清楚。在这里，我们报告了一种通过子宫内膜基质细胞原代培养方法建立的异位子宫内膜基质细胞（ESCM）的体外模型，用于研究 FKBP52 异常与子宫内膜异位症（Ems）中 PR 表达水平之间的关系的细节。同时，还通过子宫内膜异位症患者 FKBP52 和 PR 表达水平的临床研究来验证我们的结论。结果表明，子宫内膜异位症中 PR-A mRNA 和蛋白的表达水平与 FKBP52 呈正相关，FKBP52 的异常导致 PR-B mRNA 和蛋白表达减少。当 FKBP52 缺失或减少时，PR-A 和 PR-B 的 m RNA 和蛋白表达水平降低，导致异位子宫内膜细胞（ESC）的增殖和子宫内膜异位症的发生，这与临床子宫内膜异位症患者的表达水平一致，充分证实了我们的结论和模型的可靠性，对 Ems 疾病发病机制的研究和临床治疗具有重要的指导意义。
