Fotoran Wesley L, Colhone Marcelle C, Ciancaglini Pietro, Stabeli Rodrigo G, Wunderlich Gerhard
Department of Parasitology, Institute for Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
Department of Chemistry, FFCLRP-USP, University of São Paulo, Ribeirão Preto, Brazil.
ACS Biomater Sci Eng. 2016 Dec 12;2(12):2276-2286. doi: 10.1021/acsbiomaterials.6b00492. Epub 2016 Oct 17.
Liposomes loaded with GPI-anchored proteins induce a strong and effective response against protozoan pathogens when immunized in mice. On the basis of results using merozoite proteins loaded on liposomes, we tested whether a similar approach would be protective in the XL and NK65 models of lethal murine malaria infections. We also analyzed the enhancing capacity of additional adjuvants. As expected, merozoite protein loaded liposomes provided strong humoral responses (IgG titers of 10 against MSP1) in BALB/c and C57BL/6 host backgrounds and showed total protection in lethal challenges (100% survival) with , while protection against lethal challenge with NK was at least partial (11%-20% in C57BL/6 mice and no protection in BALB/c). Thus, immunization with proteoliposomes is not only highly immunogenic but also provides a protective response in widely used murine models of malaria.
负载糖基磷脂酰肌醇（GPI）锚定蛋白的脂质体在小鼠免疫时可诱导针对原生动物病原体的强烈且有效的反应。基于使用负载裂殖子蛋白的脂质体的结果，我们测试了类似方法在致死性鼠疟感染的XL和NK65模型中是否具有保护作用。我们还分析了其他佐剂的增强能力。正如预期的那样，负载裂殖子蛋白的脂质体在BALB/c和C57BL/6宿主背景中产生了强烈的体液反应（针对MSP1的IgG滴度为10），并且在用[具体病原体名称未给出]进行致死性攻击时显示出完全保护作用（100%存活），而针对NK的致死性攻击的保护作用至少是部分的（C57BL/6小鼠中为11%-20%，BALB/c小鼠中无保护作用）。因此，用蛋白脂质体免疫不仅具有高度免疫原性，而且在广泛使用的疟疾小鼠模型中提供了保护性反应。
