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使用 NMR 光谱法对 Bianchera/Belica 品种橄榄油中的橄榄苦苷进行定量分析及多酚 HPLC 分析。

Oleocanthal Quantification Using H NMR Spectroscopy and Polyphenols HPLC Analysis of Olive Oil from the Bianchera/Belica Cultivar.

机构信息

Dipartimento di Scienze Chimiche e Farmaceutiche, Università degli Studi di Trieste, via L. Giorgieri 1, 34127 Trieste, Italy.

出版信息

Molecules. 2021 Jan 5;26(1):242. doi: 10.3390/molecules26010242.

DOI:10.3390/molecules26010242
PMID:33466567
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7796514/
Abstract

The cultivar Bianchera is an autochthonous variety from the eastern part of northern Italy, but it is also cultivated in the Slovenian and Croatian peninsula of Istria where it is named Belica (Slovenia) and Bjelica (Croatia). The properties of oleocanthal, a natural anti-inflammatory ibuprofen-like compound found in commercial monocultivar extra virgin olive oils, were determined by means of both quantitative H NMR (NMR) and HPLC analyses, where NMR was identified as a rapid and reliable method for determining the oleocanthal content. The total phenolic content (TPC) was determined by means of the Folin-Ciocalteau method and the major phenols present in the olive oils were also quantified by means of HPLC analyses. All these analyses confirmed that the cultivar Bianchera was very rich in polyphenols and satisfied the health claim provided by the EU Commission Regulation on the polyphenols content of olive oils and their beneficial effects on human health.

摘要

比安切拉(Bianchera)品种是意大利北部东部的一种土生品种,但也在斯洛文尼亚和克罗地亚的伊斯特拉半岛种植,在那里它被命名为贝利卡(Belica,斯洛文尼亚)和别利卡(Bjelica,克罗地亚)。商业单品种初榨橄榄油中发现的天然抗炎布洛芬类似物油酸醇的特性通过定量核磁共振(NMR)和高效液相色谱(HPLC)分析来确定,NMR 被确定为快速可靠的油酸醇含量测定方法。总酚含量(TPC)通过福林-考尔法测定,橄榄油中存在的主要酚类物质也通过 HPLC 分析进行定量。所有这些分析都证实,比安切拉(Bianchera)品种富含多酚,符合欧盟委员会关于橄榄油多酚含量及其对人体健康有益影响的法规规定的健康声明。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/d139188a118e/molecules-26-00242-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/d139188a118e/molecules-26-00242-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/232383783002/molecules-26-00242-g005a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/236aae332ca0/molecules-26-00242-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/c4ee13862ffc/molecules-26-00242-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a29d/7796514/d139188a118e/molecules-26-00242-g013.jpg

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