换血疗法对热损伤后细胞介导免疫功能的影响。

Effect of exchange transfusion on cell-mediated immune function following thermal injury.

作者信息

Merrell S W, Shelby J, Freeman T R, Saffle J R

机构信息

Department of Surgery, University of Utah College of Medicine, Salt Lake City 84132.

出版信息

J Trauma. 1988 Feb;28(2):184-9. doi: 10.1097/00005373-198802000-00009.

DOI:10.1097/00005373-198802000-00009

PMID:3346916

Abstract

We assessed the effect of syngeneic and allogeneic exchange transfusion (XTF) on cell-mediated immunity in a murine burn model. Mice were given a 30% TBSA scald injury and lymphocyte function was monitored with the popliteal lymph node assay for host-versus-graft (HVG) or graft-versus-host (GVH) response. Nonexchanged burned animals exhibited suppression of GVH response on postburn days (PBD) 3 and 8 and suppression of HVG response on PBD 3, 8, 11, and 14. Syngeneic XTF stimulated GVH response on PBD 3, and significantly improved both GVH and HVG response to alloantigens on postburn days 3 and 8 compared to the response of burned controls. Allogeneic XTF significantly improved GVH response on PBD 3 and 8, but did not improve HVG response. Restoration of lymphocyte function in all experimental groups occurred between PBD 14 and 18 and coincided with wound healing.

摘要

我们评估了同基因和异基因换血疗法（XTF）对小鼠烧伤模型中细胞介导免疫的影响。给小鼠造成30%体表面积的烫伤，并通过腘窝淋巴结试验监测淋巴细胞功能，以检测宿主抗移植物（HVG）或移植物抗宿主（GVH）反应。未进行换血的烧伤动物在烧伤后第3天和第8天表现出GVH反应受到抑制，在烧伤后第3天、第8天、第11天和第14天表现出HVG反应受到抑制。同基因XTF在烧伤后第3天刺激了GVH反应，与烧伤对照组相比，在烧伤后第3天和第8天显著改善了对同种异体抗原的GVH和HVG反应。异基因XTF在烧伤后第3天和第8天显著改善了GVH反应，但未改善HVG反应。所有实验组的淋巴细胞功能在烧伤后第14天至第18天恢复，且与伤口愈合同时发生。