噬菌体φ6大双链RNA片段的核苷酸序列：指定病毒复制酶和转录酶的基因。
Nucleotide sequence of the large double-stranded RNA segment of bacteriophage phi 6: genes specifying the viral replicase and transcriptase.
作者信息
Mindich L, Nemhauser I, Gottlieb P, Romantschuk M, Carton J, Frucht S, Strassman J, Bamford D H, Kalkkinen N
机构信息
Department of Microbiology, Public Health Research Institute of the City of New York, Inc., New York.
出版信息
J Virol. 1988 Apr;62(4):1180-5. doi: 10.1128/JVI.62.4.1180-1185.1988.
Abstract
The genome of the lipid-containing bacteriophage phi 6 contains three segments of double-stranded RNA. We determined the nucleotide sequence of cDNA derived from the largest RNA segment (L). This segment specifies the procapsid proteins necessary for transcription and replication of the phi 6 genome. The coding sequences of the four proteins on this segment were identified on the basis of size and the correlation of predicted N-terminal amino acid sequences with those found through analysis of isolated proteins. This report completes the sequence analysis of phi 6. This constitutes the first complete sequence of a double-stranded RNA genome virus.
摘要
含脂质噬菌体φ6的基因组包含三段双链RNA。我们测定了源自最大RNA片段（L）的cDNA的核苷酸序列。该片段指定了φ6基因组转录和复制所需的原衣壳蛋白。基于大小以及预测的N端氨基酸序列与通过分离蛋白分析所发现序列的相关性，确定了该片段上四种蛋白质的编码序列。本报告完成了φ6的序列分析。这构成了双链RNA基因组病毒的首个完整序列。
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