Faculty of Chemistry, University of Warsaw, Warsaw, Poland.
Faculty of Biology, University of Warsaw, Warsaw, Poland.
J Appl Microbiol. 2021 Sep;131(3):1464-1473. doi: 10.1111/jam.15009. Epub 2021 Feb 9.
The catabolism products of the fermentation process of selected hydroxycinnamic acids initiated by different species of Lactobacillus strains were identified.
Three dietary supplements (Sanprobi IBS , BioGaia ProTectis Baby and Dicoflor 60 ) were used to isolate the Lactobacillus strains. The overnight bacterial cultures (18 h) were diluted and grown in a microaerophilic atmosphere at 37°C. Then, each phenolic acid was added to bacterial cultures and incubated for 24 h at 37°C. Samples were collected at specific intervals for a further 24 h of incubation. LC-MS/MS was used for the identification of metabolism products of selected phenolic acids.
The phenolic acids were resistant to the Lactobacillus rhamnosus GG. Lactobacillus plantarum 299v caused degradation of caffeic and ferulic acids. The former was degraded either to dihydrocaffeic acid or to 4-vinylcatechol and 4-ethylcatechol. Ferulic acid was degraded only to dihydroferulic acid. Lactobacillus reuteri DSM 17938 caused only the degradation of chlorogenic acid (5-caffeoylquinic acid, referred to IUPAC nomenclature) to caffeic acid.
Using of Lactobacilli as food additive should be taken into account that phenolic acids metabolism rate depends on not only the specific bacterial strain but also the structural properties of the acid.
鉴定由不同种属的乳酸菌发酵过程中产生的特定羟基肉桂酸的分解代谢产物。
使用三种膳食补充剂（Sanprobi IBS、BioGaia ProTectis Baby 和 Dicoflor 60）来分离乳酸菌菌株。过夜细菌培养物（18 小时）稀释后，在 37°C 的微需氧环境中生长。然后，将每种酚酸添加到细菌培养物中，并在 37°C 下孵育 24 小时。在进一步孵育 24 小时的过程中，在特定时间间隔收集样品。LC-MS/MS 用于鉴定选定酚酸的代谢产物。
酚酸对鼠李糖乳杆菌 GG 具有抗性。植物乳杆菌 299v 导致咖啡酸和阿魏酸降解。前者要么降解为二氢咖啡酸，要么降解为 4-乙烯基儿茶酚和 4-乙基儿茶酚。阿魏酸仅降解为二氢阿魏酸。雷氏乳杆菌 DSM 17938 仅导致绿原酸（IUPAC 命名法中的 5-咖啡酰奎宁酸）降解为咖啡酸。
使用乳酸菌作为食品添加剂时，应考虑到酚酸的代谢率不仅取决于特定的细菌菌株，还取决于酸的结构特性。
