通过微量滴定板酶免疫测定法检测雌二醇

Estradiol assay by microtitre plate enzyme immunoassay.

作者信息

Marcus G J, Durnford R

机构信息

Animal Research Centre, Agriculture Canada, Ottawa, Ontario.

出版信息

J Steroid Biochem. 1988 Feb;29(2):207-12. doi: 10.1016/0022-4731(88)90267-1.

DOI:10.1016/0022-4731(88)90267-1

PMID:3347060

Abstract

Development of a simple enzyme linked immunosorbent assay (ELISA) for estradiol in serum extracts is described. The assay involves use of a 96-well microtitre plate, designed for immunoassay, as the support for a purified, high-titre antiserum, raised against estradiol-6(O)-carboxymethyloxime linked to bovine serum albumin, and using horseradish peroxidase-labelled estradiol-6-(O)-carboxymethyloxime as the labelled species, with 2,2'-azino-bis-(3-ethylbenzthiazoline sulfonic acid) diammonium salt (ABTS) as the chromogenic substrate. The assay characteristics rival those of radio- or chemiluminescence immunoassays for estradiol.

摘要

本文描述了一种用于血清提取物中雌二醇的简单酶联免疫吸附测定（ELISA）方法的开发。该测定法使用专为免疫测定设计的96孔微量滴定板，作为针对与牛血清白蛋白偶联的雌二醇-6（O）-羧甲基肟产生的纯化高滴度抗血清的载体，并使用辣根过氧化物酶标记的雌二醇-6-（O）-羧甲基肟作为标记物，以2,2'-叠氮基双-（3-乙基苯并噻唑啉磺酸）二铵盐（ABTS）作为显色底物。该测定法的特性可与雌二醇的放射免疫测定或化学发光免疫测定相媲美。