Microbiology Department, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt.
J Interferon Cytokine Res. 2021 Jan;41(1):29-36. doi: 10.1089/jir.2020.0073.
Recently, studies suggested that the mesenchymal stem cells (MSCs) have anti-inflammatory and immune-modulatory roles in the induced acute lung injury in mice via controlling innate, humoral, and cell-mediated immunity. Sixty adult male mice were divided equally into three groups. Group A (control group) received an intraperitoneal (IP) phosphate-buffered saline. Group B was injected IP with lipopolysaccharide (LPS). Group C was injected IP with LPS, followed after 2 h by intravenous labeled bone marrow-derived MSCs (BM-MSCs). The plasma and bronchioalveolar lavage (BAL) fluid were collected at 12, 24, and 72 h postinjection. Estimation of total cell and neutrophils count and immunoglobulin M (IgM) in BAL fluid was performed. Enzyme-linked immunosorbent assay (ELISA) was used to analyze tumor necrosis factor-α (TNF-α) that is a proinflammatory cytokine and interleukin-10 (IL-10), which is an anti-inflammatory cytokine, in plasma. Lung samples were collected for histopathological examination at 12, 24, 72 h, and 1 week postinjection. Decreased TNF-α and increased IL-10 levels in the plasma of MSC-treated group compared to the LPS-infected group were observed. Also, decreased IgM level in BAL fluid of the MSC-treated group after 72 h compared to the LPS-infected group was detected with a resolution of inflammation and improvement in lung injury. Moreover, MSC-treated group showed a reduction in total leukocyte count and neutrophil percentage in comparison to control and LPS-infected groups. Histopathological improvement was detected in MSC-treated group as well. In conclusion, systemic MSCs injection has an anti-inflammatory and immune-modulatory effect in LPS-induced acute lung injury in mice.
最近的研究表明，间充质干细胞（MSCs）通过控制先天、体液和细胞介导的免疫，在诱导的小鼠急性肺损伤中具有抗炎和免疫调节作用。将 60 只成年雄性小鼠等分为 3 组。A 组（对照组）接受腹腔内（IP）磷酸盐缓冲盐水。B 组注射脂多糖（LPS）。C 组注射 LPS 后 2 小时静脉内标记骨髓源性间充质干细胞（BM-MSCs）。注射后 12、24 和 72 小时收集血浆和支气管肺泡灌洗液（BAL）。BAL 液中总细胞和中性粒细胞计数及免疫球蛋白 M（IgM）的测定。酶联免疫吸附试验（ELISA）用于分析血浆中促炎细胞因子肿瘤坏死因子-α（TNF-α）和抗炎细胞因子白细胞介素-10（IL-10）。注射后 12、24、72 小时和 1 周收集肺组织样本进行组织病理学检查。与 LPS 感染组相比，MSC 治疗组血浆中 TNF-α水平降低，IL-10 水平升高。注射后 72 小时，MSC 治疗组 BAL 液中 IgM 水平也较 LPS 感染组降低，炎症得到缓解，肺损伤得到改善。此外，与对照组和 LPS 感染组相比，MSC 治疗组总白细胞计数和中性粒细胞百分比降低。MSC 治疗组的组织病理学也有所改善。结论：MSC 全身注射对 LPS 诱导的小鼠急性肺损伤具有抗炎和免疫调节作用。
