Meng Yating, Jiao Yuan, Zhang Yuan, Zhang Huilin, Gong Xiaojuan, Liu Yang, Shuang Shaomin, Dong Chuan
Institute of Environmental Science, and School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
College of Environmental Science and Engineering, Taiyuan University of Technology, Jinzhong 030600, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Apr 15;251:119432. doi: 10.1016/j.saa.2021.119432. Epub 2021 Jan 6.
In this work, the red emission multifunctional carbon dots (R-CDs) were prepared via one-pot hydrothermal strategy of neutral red (NR) and ethylenediamine (EDA) for the label-free detection of berberine and curcumin, cell imaging, and fluorescent flexible film. The as-fabricated R-CDs not only possess good water dispersibility and excellent fluorescence stability, but also were successfully employed as a photoluminescent nanoprobe for label-free monitoring of berberine (BRH) and curcumin (Cur) based on dynamic quenching and internal filter effect (IFE), respectively. More importantly, as-proposed R-CDs displayed outstanding cellular permeability and lower cytotoxicity for cellular applications, which was consistent with the results of confocal fluorescence imaging and cell viability measurement of SMMC7721 cells. Thus, the multifunctional R-CDs may provide a rich tool library for biosensing and cellular imaging reagent applications. Interestingly, R-CDs were also used to manufacture R-CDs/PVA composites as fluorescent flexible films. To the best of our knowledge, this is the first demonstration of a label-free multifunctional fluorescent nanoprobe for berberine and curcumin based on red emission CDs.
在本工作中，通过中性红（NR）和乙二胺（EDA）的一锅水热法制备了红色发射多功能碳点（R-CDs），用于黄连素和姜黄素的无标记检测、细胞成像以及荧光柔性薄膜。所制备的R-CDs不仅具有良好的水分散性和优异的荧光稳定性，还分别基于动态猝灭和内滤效应（IFE）成功用作光致发光纳米探针，用于黄连素（BRH）和姜黄素（Cur）的无标记监测。更重要的是，所提出的R-CDs在细胞应用中表现出出色的细胞渗透性和较低的细胞毒性，这与SMMC7721细胞的共聚焦荧光成像和细胞活力测量结果一致。因此，多功能R-CDs可能为生物传感和细胞成像试剂应用提供丰富的工具库。有趣的是，R-CDs还被用于制造R-CDs/PVA复合材料作为荧光柔性薄膜。据我们所知，这是首次基于红色发射碳点的黄连素和姜黄素无标记多功能荧光纳米探针的演示。
