Le Minh, Penaloza Fernando, Martins Renata, Nguyen Thanh V, Nguyen Ha M, Nguyen Dang X, Nguyen Luong D, Wilting Andreas
Department of Environmental Ecology, Faculty of Environmental Science, Hanoi University of Science, VNU, Hanoi, Vietnam.
Centre for Natural Resources and Environmental Studies, VNU, Hanoi, Vietnam.
Mitochondrial DNA B Resour. 2016 Nov 22;1(1):479-482. doi: 10.1080/23802359.2016.1186520.
Mitochondrial genomes of five Laotian Rock Rat () samples from Vietnam and Laos were sequenced using an Illumina platform. After assembly, 13 protein-coding genes and two rRNA (12S and 16S) of the five genomes were aligned and analyzed with those from other related species under maximum likelihood and Bayesian inferences. Both methods revealed congruent tree topologies, which support two independently evolving clades of from Laos and Vietnam. The relaxed time calibration analysis showed that the two major lineages of the Laotian Rock Rat split about 8 million years ago, which was consistent with the results from previous studies using only cytochrome b sequences. Such a deep divergence time suggests the recognition of two rock rat species, but further nuclear DNA and morphological data are needed to solve the taxonomy of this taxon.
使用Illumina平台对来自越南和老挝的5个老挝岩鼠样本的线粒体基因组进行了测序。组装后，将这5个基因组的13个蛋白质编码基因和两个rRNA（12S和16S）与其他相关物种的相应基因进行比对，并在最大似然法和贝叶斯推断下进行分析。两种方法都揭示了一致的系统发育树拓扑结构，支持来自老挝和越南的老挝岩鼠的两个独立进化分支。宽松的时间校准分析表明，老挝岩鼠的两个主要谱系大约在800万年前分化，这与之前仅使用细胞色素b序列的研究结果一致。如此深的分化时间表明应识别出两种岩鼠物种，但需要进一步的核DNA和形态学数据来解决该分类单元的分类问题。
