一种特异性抗血清可识别可乐定置换物质：对大脑自身可乐定结构的启示。

A specific antiserum recognizes clonidine-displacing substance: implications for the structure of the brain's own clonidine.

作者信息

Meeley M P, Towle A C, Ernsberger P, Reis D J

机构信息

Division of Neurobiology, Cornell University Medical College, New York, NY 10021.

出版信息

Neurosci Lett. 1988 Jan 11;84(1):84-90. doi: 10.1016/0304-3940(88)90342-4.

DOI:10.1016/0304-3940(88)90342-4

PMID:3347376

Abstract

A polyclonal antiserum was raised in rabbit against the clonidine analog p-aminoclonidine (PAC) coupled to hemocyanin. The antiserum (anti-PAC3) exhibited high affinity for unconjugated [3H]PAC (Kd 0.32 +/- 0.07 nM) in a rapid-filtration radioimmunoassay. Competition experiments showed that PAC, clonidine, and naphazoline cross-reacted with the anti-PAC3 antiserum, whereas a number of other structurally related compounds did not. An endogenous clonidine-displacing substance (CDS) partially purified from bovine brain also inhibited specific [3H]PAC binding to anti-PAC3 in a dose-dependent manner. Thus, (a) anti-PAC3 antiserum is specific for clonidine and closely related compounds, and (b) CDS may structurally resemble clonidine since it is recognized by this highly specific antiserum.

摘要

用与血蓝蛋白偶联的可乐定类似物对氨基可乐定（PAC）在兔体内制备了多克隆抗血清。在快速过滤放射免疫分析中，该抗血清（抗PAC3）对未结合的[3H]PAC表现出高亲和力（解离常数Kd为0.32±0.07 nM）。竞争实验表明，PAC、可乐定和萘甲唑啉与抗PAC3抗血清发生交叉反应，而其他一些结构相关化合物则不发生。从牛脑中部分纯化的内源性可乐定置换物质（CDS）也以剂量依赖性方式抑制[3H]PAC与抗PAC3的特异性结合。因此，（a）抗PAC3抗血清对可乐定和密切相关的化合物具有特异性，（b）CDS在结构上可能类似于可乐定，因为它能被这种高度特异性的抗血清识别。