斯里兰卡原鸡的完整线粒体基因组及系统发育研究

Complete mitochondrial genome of Sri Lankan Junglefowl () and phylogenetic study.

作者信息

Hirimuthugoda Nalini Yasoda, Adeola Adeniyi C, Chen Xing, Perera Patthamesthrige Wimal Anthony, Gunawardena Weligalle Wedarallage Dewar Asoka, Gunwardana Humpita Gamaralalage Thilini Nisanka, Yin Ting-Ting, Wang Ming-Shan, Li Gui-Mei, Peng Min-Sheng, Zhang Ya-Ping

机构信息

Department of Animal Science, Faculty of Agriculture, University of Ruhuna, Matara, Sri Lanka.

Yunnan Laboratory of Molecular Biology of Domestic Animals, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China.

出版信息

Mitochondrial DNA B Resour. 2018 Jan 5;3(1):83-84. doi: 10.1080/23802359.2017.1422409.

DOI:10.1080/23802359.2017.1422409

PMID:33474074

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7799929/

Abstract

The complete mitochondrial genomes of two Sri Lankan junglefowl (: CJF) individuals were sequenced by using next-generation sequencing technique. Samples were collected from Rathnapura and Pelmadulla areas in Sri Lanka. The complete mitochondrial DNA is 16,839 bp in length, with a typical mitogenome structure composed of a non-coding control region, 22 tRNA, two rRNA, and 13 protein-coding genes. Overall base composition is 30% A, 23.9% T, 32.3% C, and 13.6% G indicating high content of 54.0% A + T for both individuals. Phylogenetic analysis reveals that CJF samples cluster with the clade of the green junglefowl () and red junglefowl () than to grey junglefowl (: GyJF). This result can be subsequently used to provide essential information for junglefowl evolution.

摘要

利用下一代测序技术对两只斯里兰卡原鸡（: CJF）个体的完整线粒体基因组进行了测序。样本采集于斯里兰卡的拉特纳普勒和佩尔马杜拉地区。完整的线粒体DNA长度为16,839 bp，具有典型的线粒体基因组结构，由一个非编码控制区、22个tRNA、两个rRNA和13个蛋白质编码基因组成。总体碱基组成为30%A、23.9%T、32.3%C和13.6%G，表明两个个体的A+T含量均高达54.0%。系统发育分析表明，CJF样本与绿原鸡（）和红原鸡（）的分支聚类，而不是与灰原鸡（: GyJF）聚类。这一结果随后可用于为原鸡进化提供重要信息。