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利用陈旧的动物标本样本,通过线粒体基因(12SrRNA和细胞色素b)对猫属(哺乳纲:食肉目:猫科)的物种和亚种进行法医信息核苷酸测序(FINS)。

Forensically informative nucleotide sequencing (FINS) for species and subspecies of genus (Mammalia: Carnivora: Felidae) through mitochondrial genes (12SrRNA and cytochrome b) by using old taxidermy samples.

作者信息

Bahuguna Archana

机构信息

Molecular Systematic Laboratory, Northern Regional Centre, Zoological Survey of India, Dehradun, India.

出版信息

Mitochondrial DNA B Resour. 2018 May 17;3(2):615-619. doi: 10.1080/23802359.2018.1462115.

Abstract

Genus comprises four species, i.e. Leopard cat, Rusty spotted cat, Fishing cat, Flat-headed cat, listed under IUCN as threatened species except In U.S.A., is listed as Endangered. Subspecies of , i.e. is listed as Critically Endangered under IUCN since 2008. The present study describes the use of two markers 12SrRNA and cytochrome b genes to differentiate the three species and three subspecies of Old taxidermy samples (three skin samples) of and were used from India for the study. The study done by using DNAsp v5, MEGA 6.0, and Network 5.0.0.1, proved that both gene markers are useful for differentiating the species and subspecies of . This study is also the first study to present forensically informative nucleotide sequence (FINS) for three species and three subspecies of .

摘要

豹猫属包含四个物种,即豹猫、锈斑豹猫、渔猫、扁头豹猫,除在美国被列为濒危物种外,在国际自然保护联盟(IUCN)均被列为受威胁物种。豹猫属的亚种,即自2008年以来在IUCN被列为极度濒危物种。本研究描述了使用12SrRNA和细胞色素b基因这两种标记物来区分豹猫属的三个物种和三个亚种。研究使用了来自印度的豹猫属和渔猫的旧标本样本(三个皮肤样本)。通过使用DNAsp v5、MEGA 6.0和Network 5.0.0.1进行的研究证明,这两种基因标记物都有助于区分豹猫属的物种和亚种。本研究也是首次针对豹猫属的三个物种和三个亚种提供法医信息核苷酸序列(FINS)。

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