Department of Biology, Wilfrid Laurier University, Waterloo, ON, Canada.
Department of Health Sciences, Wilfrid Laurier University, Waterloo, ON, Canada.
In Vitro Cell Dev Biol Anim. 2021 Jan;57(1):86-94. doi: 10.1007/s11626-020-00536-4. Epub 2021 Jan 20.
The goal of the present study was to characterize a novel bovine intestinal myofibroblast (BT-IMF) cell line isolated from a fetal bovine intestine. This cell type is of importance as intestinal myofibroblasts play a key role in controlling intestinal epithelial cell proliferation, intestinal regulation, wound healing, epithelial cell turnover, and structural support. The present work demonstrates that BT-IMF cells could be successfully cryopreserved and thawed and cultured past 25 passages. Immunocytochemical staining of the BT-IMF cell line was positive for vimentin and smooth muscle actin (α-SMA) and negative for pancytokeratin, suggesting that the cells are myofibroblastic in type. Growth kinetic experiments demonstrate that hydrocortisone negatively impacts BT-IMF growth and non-essential amino acids enhance its proliferation. Inosine monophosphate (IMP) is a dietary nucleotide and is essential for supporting animal health. Stimulation with IMP bound to a novel phytoglycogen-based nanocarrier (IMP-NP) showed enhanced cell proliferation. BT-IMF provides a new tool for studying bovine cells in vitro and may be of particular interest for cultured meat manufacturing in the future.
本研究的目的是从胎牛肠中分离出一种新型的牛肠肌成纤维细胞（BT-IMF）细胞系，并对其进行鉴定。肠肌成纤维细胞在控制肠道上皮细胞增殖、肠道调节、伤口愈合、上皮细胞更新和结构支撑方面发挥着关键作用，因此该细胞类型具有重要意义。本研究表明，BT-IMF 细胞可以成功地进行冷冻保存和复苏，并在 25 代以上的培养中存活。BT-IMF 细胞系的免疫细胞化学染色呈波形蛋白和平滑肌肌动蛋白（α-SMA）阳性，细胞角蛋白阴性，提示该细胞为肌成纤维细胞类型。生长动力学实验表明，氢化可的松对 BT-IMF 的生长具有负向影响，非必需氨基酸可增强其增殖。肌苷单磷酸（IMP）是一种膳食核苷酸，对维持动物健康至关重要。用与新型植物糖蛋白基纳米载体（IMP-NP）结合的 IMP 进行刺激，显示出增强的细胞增殖。BT-IMF 为体外研究牛细胞提供了一种新工具，将来可能对培养肉制造特别感兴趣。
