Svistushkin V M, Timashev P S, Shekhter A B, Zolotova A V, Mokoyan Zh T, Svistushkin M V
I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
Institute for Regenerative Medicine of the I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
Vestn Otorinolaringol. 2020;85(6):23-26. doi: 10.17116/otorino20208506123.
The aim of our study was an experimental evaluation of tissue engineering approach to chronic tympanic membrane perforation closure.
Chronic tympanic membrane perforation models were created both sides in 12 chinchillas. Right sided perforations were divided into two equal groups (A and B) according to treatment; left sided perforations were used as a control group. Group A perforations were treated with collagen scaffold and fibroblast growth factor, group B perforations were treated with collagen scaffold only. During follow-up, we provided otovideoendoscopy for closure rates assessement every 2 weeks. In case of perforation closure, a morphological investigation of the regenerate was performed.
Group A perforations were totally closed 2 weeks after treatment in all animals. In group B, complete closure of perforation was achieved after the third treatment procedure in one case. There were no spontaneous perforation closure in the control group. According to morphological investigation, the restoration of trilaminar structure was observed only in tissue engineering group tympanic membranes.
本研究旨在对慢性鼓膜穿孔闭合的组织工程方法进行实验评估。
在12只龙猫双侧建立慢性鼓膜穿孔模型。右侧穿孔根据治疗方法分为两个相等的组（A组和B组）；左侧穿孔用作对照组。A组穿孔用胶原支架和成纤维细胞生长因子治疗，B组穿孔仅用胶原支架治疗。在随访期间，每2周进行一次耳内镜检查以评估闭合率。如果穿孔闭合，则对再生组织进行形态学研究。
所有动物的A组穿孔在治疗后2周完全闭合。B组中，1例在第三次治疗后实现了穿孔的完全闭合。对照组没有自发穿孔闭合。根据形态学研究，仅在组织工程组的鼓膜中观察到三层结构的恢复。
