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[Mycobacterium szulgai as positive control helps to detect contaminations in the detection of Mycobacterium tuberculosis-complex in formalin fixed, paraffin embedded tissues by 16SrDNA PCR].

作者信息

Goldmann Torsten, Hillemann Doris, Maurer Florian, Kalsdorf Barbara, Krupar Rosemarie, Stellmacher Florian, Perner Sven

机构信息

Pathologie, Forschungszentrum Borstel, Leibniz Lungenzentrum, Parkallee 1-40, 23845, Borstel, Deutschland.

Airway Research Center North, German Center for Lung Research, 22927, Großhansdorf, Deutschland.

出版信息

Pathologe. 2021 Feb;42(1):83-85. doi: 10.1007/s00292-021-00912-1. Epub 2021 Jan 21.

DOI:10.1007/s00292-021-00912-1
PMID:33475807
Abstract

The detection of Mycobacterium tuberculosis complex DNA by PCR using formalin-fixed paraffin-embedded material has become an integral part of molecular-pathological diagnostics. We describe an approach that enables the detection of contamination by using Mycobacterium szulgai as a positive control, contributing to the reduction of false-positive results.

摘要

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本文引用的文献

1
Inter-laboratory validation of PCR-based detection of Mycobacterium tuberculosis in formalin-fixed, paraffin-embedded tissues.基于聚合酶链反应（PCR）的福尔马林固定石蜡包埋组织中结核分枝杆菌检测的实验室间验证
Virchows Arch. 2005 Sep;447(3):573-85. doi: 10.1007/s00428-005-1233-3. Epub 2005 Jun 21.