Gonçalves Jenifer Pendiuk, de Oliveira Carolina Camargo, da Silva Trindade Edvaldo, Riegel-Vidotti Izabel Cristina, Vidotti Marcio, Simas Fernanda Fogagnoli
Laboratory of Inflammatory and Neoplastic Cells/Laboratory of Sulfated Polysaccharides Investigation, Cell Biology Department, Section of Biological Sciences, Universidade Federal do Paraná (UFPR), Av Cel Francisco H dos Santos, s/n, CEP 81530-980 Curitiba, PR, Brazil.
Macromolecules and Interfaces Research Group, Department of Chemistry, UFPR, Av Cel Francisco H dos Santos, s/n, CEP 81530-980 Curitiba, PR, Brazil.
Int J Biol Macromol. 2021 Mar 15;173:109-117. doi: 10.1016/j.ijbiomac.2021.01.101. Epub 2021 Jan 18.
Although polyaniline (PANI) is a widely investigated conductive polymer for biological applications, studies addressing the biocompatibility of colloidal PANI dispersions are scarcely found in the literature of the area. Therefore, PANI nanoparticles stabilized by the natural polysaccharide gum Arabic (GA) were screened for their biocompatibility. The GA successfully stabilized the colloidal PANI-GA dispersions when exposed to a protein-rich medium, showing compatibility with the biological environment. The results obtained from a series of in vitro assays showed that, after up to 48 h of exposure to a range of PANI-GA concentrations (1-50 μg/mL), both mouse BALB/3T3 fibroblasts and RAW 264.7 macrophages showed no evidence of change in cellular proliferation, viability and metabolic activity. An increase in macrophage granularity poses as evidence of phagocytic uptake of PANI-GA, without resulting activation of this cell type. Additionally, the PANI-GA nanoparticles modulated the cell morphology changes induced on fibroblasts by GA in a concentration-dependent manner. Thus, this unprecedented biocompatibility study of PANI nanoparticles stabilized by a plant gum exudate polysaccharide showed promising results. This simple biomaterial might be further developed into colloidal formulations for biological and biomedical applications, taking advantage of its versatility, biocompatibility, and conductive properties.
尽管聚苯胺（PANI）是一种在生物应用领域得到广泛研究的导电聚合物，但在该领域的文献中几乎找不到关于胶体聚苯胺分散体生物相容性的研究。因此，对由天然多糖阿拉伯胶（GA）稳定的聚苯胺纳米颗粒的生物相容性进行了筛选。当暴露于富含蛋白质的介质中时，GA成功地稳定了胶体聚苯胺 - GA分散体，显示出与生物环境的相容性。一系列体外试验的结果表明，在暴露于一系列聚苯胺 - GA浓度（1 - 50μg/mL）长达48小时后，小鼠BALB/3T3成纤维细胞和RAW 264.7巨噬细胞均未显示出细胞增殖、活力和代谢活性有变化的迹象。巨噬细胞颗粒度的增加表明存在对聚苯胺 - GA的吞噬摄取，但并未导致这种细胞类型的激活。此外，聚苯胺 - GA纳米颗粒以浓度依赖的方式调节了GA对成纤维细胞诱导的细胞形态变化。因此，这项对由植物胶渗出多糖稳定的聚苯胺纳米颗粒进行的前所未有的生物相容性研究显示出了有前景的结果。这种简单的生物材料可能会利用其多功能性、生物相容性和导电特性，进一步开发成用于生物和生物医学应用的胶体配方。
