Zhang Xiao-Qin, Zhang Yuan-Ling, Li Bing-Yan, Feng Ya-Nan, Li Ping, Zhang Dong-Sheng, Wang Li-Wei, Hao Xing-Yu
College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China.
Ying Yong Sheng Tai Xue Bao. 2021 Jan;32(1):182-190. doi: 10.13287/j.1001-9332.202101.022.
The climate change caused by elevated CO concentration and drought are bound to affect the growth of soybean. Few studies have addressed the effects of elevated CO concentration on the physiology and biochemistry of soybean under drought stress. Here, we examined the changes of photosynthetic ability, photosynthetic pigment accumulation, antioxidant level, osmotic adjustment substances, hormone levels, signal transduction enzymes and gene expression level of soybean at flowering stage under different CO concentration (400 and 600 μmol·mol) and drought stress (normal water: leaf relative water content was 83%-90%; drought stress: leaf relative water content was 64%-70%). The results showed that the transpiration rate, water use efficiency and net photosynthetic rate of soybean leaves were significantly increased by elevated CO concentration, but the content of chlorophyll b was decreased under drought stress. Elevated CO concentration significantly increased peroxidase activity and abscisic acid content of leaves under drought stress, decreased the content of proline, and did not affect the content of soluble saccharides. The increased CO concentration under drought stress significantly promoted the content of calcium-dependent protein kinase and glutathione-S-transferase, and up-regulated the expression of related genes, while significantly decreased the content of mitogen-activated protein kinase and the heat shock protein, and down-regulated the expression of their genes. The results would be helpful to understand the impacts of climate change on the growth, physiology and biochemistry of soybean, and to deal with the production problems of soybean under future climate change.
二氧化碳浓度升高和干旱导致的气候变化必然会影响大豆的生长。很少有研究探讨二氧化碳浓度升高对干旱胁迫下大豆生理生化的影响。在此，我们研究了不同二氧化碳浓度（400和600 μmol·mol）和干旱胁迫（正常水分：叶片相对含水量为83%-90%；干旱胁迫：叶片相对含水量为64%-70%）下大豆开花期光合能力、光合色素积累、抗氧化水平、渗透调节物质、激素水平、信号转导酶和基因表达水平的变化。结果表明，二氧化碳浓度升高显著提高了大豆叶片的蒸腾速率、水分利用效率和净光合速率，但干旱胁迫下叶绿素b含量降低。二氧化碳浓度升高显著提高了干旱胁迫下叶片的过氧化物酶活性和脱落酸含量，降低了脯氨酸含量，对可溶性糖含量无影响。干旱胁迫下二氧化碳浓度升高显著促进了钙依赖蛋白激酶和谷胱甘肽-S-转移酶的含量，并上调了相关基因的表达，同时显著降低了丝裂原活化蛋白激酶和热休克蛋白的含量，并下调了其基因的表达。这些结果将有助于理解气候变化对大豆生长、生理生化的影响，并应对未来气候变化下大豆的生产问题。
