Research Division of Radiation Science, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeougeup 56212, Korea.
Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea.
Molecules. 2021 Jan 18;26(2):476. doi: 10.3390/molecules26020476.
, a protein-rich edible insect, is widely used as a traditional medicine in China. Several pharmacological studies have reported the anticancer activity of extracts; however, the capacity of extracts as immune potentiators for increasing the efficacy of cancer immunotherapy is still unverified. In the present study, we investigated the immunomodulatory role of protein-rich extract (BBPE) in bone marrow-derived dendritic cells (BMDCs) and DC vaccine-immunized mice. BBPE-treated BMDCs displayed characteristics of mature immune status, including high expression of surface molecules (CD80, CD86, major histocompatibility complex (MHC)-I, and MHC-II), increased production of proinflammatory cytokines (tumor necrosis factor-α and interleukin-12p70), enhanced antigen-presenting ability, and reduced endocytosis. BBPE-treated BMDCs promoted naive CD4 and CD8 T-cell proliferation and activation. Furthermore, BBPE/ovalbumin (OVA)-pulsed DC-immunized mice showed a stronger OVA-specific multifunctional T-cell response in CD4 and CD8 T cells and a stronger Th1 antibody response than mice receiving differently treated DCs, which showed the enhanced protective effect against tumor growth in E.G7 tumor-bearing mice. Our data demonstrate that BBPE can be a novel immune potentiator for a DC-based vaccine in anticancer therapy.
黄粉虫，一种富含蛋白质的食用昆虫，在中国被广泛用作传统药物。几项药理学研究报告了 提取物的抗癌活性；然而， 提取物作为免疫增强剂来提高癌症免疫治疗的疗效尚未得到验证。在本研究中，我们研究了富含蛋白质的黄粉虫提取物（BBPE）在骨髓来源的树突状细胞（BMDC）和 DC 疫苗免疫小鼠中的免疫调节作用。BBPE 处理的 BMDC 表现出成熟免疫状态的特征，包括表面分子（CD80、CD86、主要组织相容性复合体（MHC）-I 和 MHC-II）的高表达、促炎细胞因子（肿瘤坏死因子-α和白细胞介素-12p70）的产生增加、抗原呈递能力增强和内吞作用减少。BBPE 处理的 BMDC 促进了幼稚 CD4 和 CD8 T 细胞的增殖和活化。此外，BBPE/卵清蛋白（OVA）脉冲 DC 免疫的小鼠在 CD4 和 CD8 T 细胞中表现出更强的 OVA 特异性多功能 T 细胞反应和更强的 Th1 抗体反应，比接受不同处理的 DC 免疫的小鼠更强，这表明其在 E.G7 荷瘤小鼠中增强了对肿瘤生长的保护作用。我们的数据表明，BBPE 可以成为基于 DC 的癌症治疗中免疫增强剂的新型免疫增强剂。
