基于无溶剂脂质双层微阵列的跨膜hERG通道电流的并行记录

Parallel Recordings of Transmembrane hERG Channel Currents Based on Solvent-Free Lipid Bilayer Microarray.

作者信息

Miyata Ryusuke, Tadaki Daisuke, Yamaura Daichi, Araki Shun, Sato Madoka, Komiya Maki, Ma Teng, Yamamoto Hideaki, Niwano Michio, Hirano-Iwata Ayumi

机构信息

Laboratory for Nanoelectronics and Spintronics, Research Institute of Electrical Communication, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.

Advanced Institute for Materials Research, Tohoku University, 2-1-1 Katahira, Aoba-ku, Sendai 980-8577, Japan.

出版信息

Micromachines (Basel). 2021 Jan 19;12(1):98. doi: 10.3390/mi12010098.

DOI:10.3390/mi12010098

PMID:33478052

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7835820/

Abstract

The reconstitution of ion-channel proteins in artificially formed bilayer lipid membranes (BLMs) forms a well-defined system for the functional analysis of ion channels and screening of the effects of drugs that act on these proteins. To improve the efficiency of the BLM reconstitution system, we report on a microarray of stable solvent-free BLMs formed in microfabricated silicon (Si) chips, where micro-apertures with well-defined nano- and micro-tapered edges were fabricated. Sixteen micro-wells were manufactured in a chamber made of Teflon, and the Si chips were individually embedded in the respective wells as a recording site. Typically, 11 to 16 BLMs were simultaneously formed with an average BLM number of 13.1, which corresponded to a formation probability of 82%. Parallel recordings of ion-channel activities from multiple BLMs were successfully demonstrated using the human related gene (hERG) potassium channel, of which the relation to arrhythmic side effects following drug treatment is well recognized.

摘要

在人工形成的双层脂质膜（BLM）中重构离子通道蛋白，为离子通道的功能分析以及筛选作用于这些蛋白的药物效果提供了一个明确的系统。为提高BLM重构系统的效率，我们报道了一种在微加工硅（Si）芯片中形成的稳定无溶剂BLM微阵列，其中制造了具有明确纳米和微锥形边缘的微孔。在由特氟龙制成的腔室中制造了16个微孔，并且将Si芯片作为记录位点分别嵌入各个孔中。通常，同时形成11至16个BLM，平均BLM数量为13.1，形成概率为82％。使用人类相关基因（hERG）钾通道成功证明了从多个BLM并行记录离子通道活性，药物治疗后其与心律失常副作用的关系已得到充分认识。