Hirano-Iwata Ayumi, Oshima Azusa, Mozumi Hideki, Kimura Yasuo, Niwano Michio
Graduate School of Biomedical Engineering, Tohoku University, Aoba, Aramaki, Sendai, Miyagi, Japan.
Anal Sci. 2012;28(11):1049-57. doi: 10.2116/analsci.28.1049.
In this review, we will discuss our recent approaches for the formation of mechanically stable bilayer lipid membranes (BLMs) by combining with BLM formation and micro- and nano-fabrication techniques. BLMs were prepared across a microaperture fabricated in silicon (Si) chips or nanoporous alumina films using a minimized amount of organic solvent. Although BLMs spanned over the porous alumina film showed better electrical properties, such as background current noise and current transient, BLMs suspended in a thin Si(3)N(4) septum showed a much superior BLM stability. The BLMs showed tolerance to a high voltage of ±1 V, a membrane lifetime of >40 h, and tolerance to repetitive solution exchanges. Application to a drug screening system has been examined by using the human ether-a-go-go-related gene (hERG) potassium channel as an illustrative example. The potentiality of the present system as a platform of the high-throughput analysis for ion-channel protein is also discussed.
在本综述中，我们将讨论我们最近通过结合双层脂质膜（BLM）形成技术以及微纳制造技术来制备机械稳定的BLM的方法。使用最少的有机溶剂，在硅（Si）芯片或纳米多孔氧化铝膜中制造的微孔上制备BLM。尽管跨多孔氧化铝膜形成的BLM表现出更好的电学性质，如背景电流噪声和电流瞬变，但悬浮在薄Si(3)N(4)隔膜中的BLM表现出更优异的稳定性。这些BLM能够耐受±1 V的高电压，膜寿命超过40小时，并且耐受重复的溶液交换。以人类醚 - 去极化相关基因（hERG）钾通道为例，研究了其在药物筛选系统中的应用。还讨论了本系统作为离子通道蛋白高通量分析平台的潜力。
