Tobolski Dawid, Łukasik Karolina, Bacławska Agnieszka, Skarżyński Dariusz Jan, Hostens Miel, Barański Wojciech
Department of Internal Diseases with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 14, 10-957 Olsztyn, Poland.
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland.
Animals (Basel). 2021 Jan 19;11(1):236. doi: 10.3390/ani11010236.
After parturition, the uterus undergoes significant reconstruction, allows the endometrium to create an environment for subsequent embryo development. Here, we used an unsupervised algorithmic approach to select characteristic endometrial mRNA expression patterns of proposed markers and investigate each marker's role as an individual indicator of reproductive success. Clinically healthy cows at a sixth week postpartum were examined, the percentage of neutrophils (PMNs%) in the cytological smear was calculated, and an endometrial biopsy was taken for qPCR. Based on pregnancy examination, cows were divided into three groups: Pregnant before 100 days postpartum (P100, n = 11), pregnant between 100-200-day (P200, n = 14), and culled (C, n = 10). Animals were also classified based on two PMNs% thresholds > 5% PMNs and > 10% PMNs. The expression of and was higher in >10%PMNs. The expression of PTGS1 was higher in the P200 compared to P100. Upregulation of inhibin A subunit () and downregulation of inhibin β A subunit () were observed in the P100. ratio was the most accurate linear predictor of the calving-to-conception interval. The application of the k-means algorithm allowed the identification of five unique expression patterns. The sensitivity and specificity of predicting allocation to P100 were 81% and 79%. We also documented the low efficiency of genes associated with subclinical endometritis and PMNs% in determining reproductive capability. These results suggested the presence of distinctive expression patterns in 6 weeks postpartum, correlated with cows' reproductive capacity. Furthermore, we proposed the ratio as an indicator of calving-to-conception interval length.
分娩后，子宫会经历显著的重建过程，使子宫内膜为后续胚胎发育创造环境。在此，我们采用无监督算法方法来选择所提出标记物的特征性子宫内膜mRNA表达模式，并研究每个标记物作为生殖成功个体指标的作用。对产后六周临床健康的奶牛进行检查，计算细胞学涂片中性粒细胞百分比（PMNs%），并采集子宫内膜活检样本进行qPCR检测。根据妊娠检查，奶牛被分为三组：产后100天内怀孕（P100，n = 11）、怀孕100 - 200天（P200，n = 14）和淘汰（C，n = 10）。动物还根据两个PMNs%阈值（> 5% PMNs和> 10% PMNs）进行分类。在> 10% PMNs组中，[具体基因1]和[具体基因2]的表达较高。与P100相比，P200组中PTGS1的表达较高。在P100组中观察到抑制素A亚基（[具体基因3]）上调和抑制素βA亚基（[具体基因4]）下调。[具体基因5]比值是产犊至受孕间隔最准确的线性预测指标。k均值算法的应用使得能够识别出五种独特的表达模式。预测分配到P100组的敏感性和特异性分别为81%和79%。我们还记录了与亚临床子宫内膜炎相关的基因和PMNs%在确定生殖能力方面的低效率。这些结果表明产后6周存在独特的表达模式，与奶牛的生殖能力相关。此外，我们提出[具体基因5]比值作为产犊至受孕间隔长度的指标。
