Xinjiang Medical University College of Pharmacy, China.
Central Laboratory of Xinjiang Medical University, China.
Folia Neuropathol. 2020;58(4):365-376. doi: 10.5114/fn.2020.102439.
We aimed to explore the role of a novel traditional herbal compound medicine (HCM) in Alzheimer's disease (AD).
72 rats were randomized into control, AD, Donepezil and HCM groups. Injection of -amyloid peptide (A1-42) into the lateral ventricle was used to induce AD in rats. Rats in treatment groups received HCM (1.5, 3.0 and 6.0 g/kg) and Donepezil (0.92 mg/kg) for 21 days, respectively. The spatial learning and memory ability were observed by Morris water maze (MWM) test. Haematoxylin-eosin (HE) staining was carried out for pathological morphology. The contents of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) in the hippocampus were determined using the spectrophotometric method. A expression was measured by immunohistochemistry and Western blotting.
Rats in HCM groups spent less time to locate the platform and performed better in spatial learning and memory than the AD group (p < 0.05). Hippocampus in the HCM (6.0 g/kg) group had a complete pyramidal cell layer, in which the structure of morphology was normal and the number of neurons was larger than in the AD group (p < 0.01). The contents of SOD, CAT, GSH-Px were notably increased and MDA content was significantly decreased in the hippocampus in HCM groups than in the AD group (p < 0.01). The expression levels of A1-42 in HCM groups were markedly decreased than in the AD group (p < 0.01).
HCM has a protective effect on the learning and memory capacity in AD in rats, indicating that HCM had cognitive enhancing potentials on AD.
本研究旨在探讨一种新型的传统中草药复合药物（HCM）在阿尔茨海默病（AD）中的作用。
72 只大鼠随机分为对照组、AD 组、多奈哌齐组和 HCM 组。向大鼠侧脑室注射β-淀粉样肽（Aβ1-42）诱导 AD。治疗组大鼠分别给予 HCM（1.5、3.0 和 6.0 g/kg）和多奈哌齐（0.92 mg/kg）治疗 21 天。采用 Morris 水迷宫（MWM）试验观察大鼠的空间学习记忆能力。采用苏木精-伊红（HE）染色观察病理形态。采用分光光度法测定海马中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）和丙二醛（MDA）的含量。采用免疫组化和 Western blot 法检测 Aβ的表达。
与 AD 组相比，HCM 组大鼠找到平台的时间更短，空间学习和记忆能力更好（p<0.05）。HCM（6.0 g/kg）组大鼠海马锥体细胞层完整，形态结构正常，神经元数量多于 AD 组（p<0.01）。与 AD 组相比，HCM 组大鼠海马 SOD、CAT、GSH-Px 含量明显升高，MDA 含量明显降低（p<0.01）。与 AD 组相比，HCM 组大鼠海马 Aβ1-42 表达水平明显降低（p<0.01）。
HCM 对 AD 大鼠的学习记忆能力具有保护作用，提示 HCM 对 AD 具有认知增强作用。
