State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
J Histotechnol. 2021 Jun;44(2):99-110. doi: 10.1080/01478885.2020.1861908. Epub 2021 Jan 22.
The A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family is gradually being recognized as an important family of mediators that, along with the matrix metalloproteinases (MMPs), control the degradation process in osteoarthritis (OA). The objective of this study was to uncover the detailed alterations of ADAMTS1, ADAMTS2, and ADAMTS5 in the knee joint of OA mice. The OA model was established by anterior cruciate ligament transection (ACLT) on the knee joints of C57BL/6 J mice. The mice showed representative phenotypes of ACLT-induced OA, including obvious deterioration of the cartilage, reductions in the collagen and proteoglycan components in the cartilage matrix of OA mice, and increased inflammation and osteoclast activity. By qPCR, the gene expression levels of were the top-ranked among in cartilage/chondrocytes, osteogenic tissue/osteoblasts, and cortical bone/osteocytes. Moreover, the protein expression levels of ADAMTS1, -2, and -5 were all increased in articular cartilage, the growth plate, and subchondral bone of the knee joint. The results suggest the important roles of ADAMTS1, -2, and -5 in OA disease, which will be helpful in further research on degenerative changes in OA.
解整合素金属蛋白酶与凝血酶样金属蛋白酶（ADAMTS）家族逐渐被认为是重要的介质家族，与基质金属蛋白酶（MMPs）一起控制骨关节炎（OA）中的降解过程。本研究旨在揭示 ADAMTS1、ADAMTS2 和 ADAMTS5 在 OA 小鼠膝关节中的详细变化。通过对 C57BL/6J 小鼠膝关节前交叉韧带切断（ACLT）建立 OA 模型。这些小鼠表现出 ACLT 诱导的 OA 的代表性表型，包括软骨明显恶化、OA 小鼠软骨基质中胶原和蛋白聚糖成分减少以及炎症和破骨细胞活性增加。通过 qPCR， 在软骨/软骨细胞、成骨组织/成骨细胞和皮质骨/骨细胞中， 的基因表达水平排名最高。此外，ADAMTS1、-2 和 -5 的蛋白表达水平均在膝关节关节软骨、生长板和软骨下骨中增加。这些结果表明 ADAMTS1、-2 和 -5 在 OA 疾病中具有重要作用，这将有助于进一步研究 OA 的退行性变化。
