Royal Veterinary College, University of London, London, UK NW1 0TU,
Wildlife Health Services, Zoological Society of London, ZSL London Zoo, Regent's Park, London NW1 4RY, UK.
J Zoo Wildl Med. 2020 Nov;51(3):591-597. doi: 10.1638/2019-0160.
Fifty-eight frozen postmortem lung and air sac samples were collected from penguins housed at 21 zoological collections throughout the United Kingdom, from 2007 to 2018. , a significant respiratory pathogen of penguins in captivity, was isolated from 15 of the 22 penguins with gross lesions. Of the penguins with gross lesions of aspergillosis at postmortem examination, the pathogen was cultured from 63.6% (15 of 22) of frozen samples. was cultured from 2.7% (1 of 36) of tissues collected from penguins without gross lesions at postmortem. Additionally, of 18 fresh samples that cultured at the time of postmortem, 15 samples (83%) yielded isolates that were successfully cultured from frozen tissue. Minimum inhibitory concentration (MIC) data demonstrated that all isolates were susceptible to terbinafine and voriconazole, and all were resistant to itraconazole, using published MIC cutoff values. Comparison isolates from fresh tissues had identical antimicrobial susceptibility to isolates from the same tissues after being frozen. This study demonstrates that can be isolated from frozen respiratory tissues of penguins, even after freezing for more than 10 yr.
从 2007 年至 2018 年，从英国 21 个动物园收集了 58 个冷冻的死后肺和气囊样本，这些样本来自于 21 个动物园的企鹅。烟曲霉是圈养企鹅的一种重要呼吸道病原体，从 22 只有大体病变的企鹅中分离出了 15 株。在死后检查有曲霉菌病大体病变的企鹅中，63.6%（22 只中的 15 只）的冷冻样本培养出了病原体。在死后未发现大体病变的企鹅的组织中，从 2.7%（36 只中的 1 只）的组织中培养出了 。此外，在死后进行培养时，18 个新鲜样本中，有 15 个样本（83%）培养出的分离株可成功从冷冻组织中培养出来。最低抑菌浓度（MIC）数据表明，所有分离株均对特比萘芬和伏立康唑敏感，且均对伊曲康唑耐药，使用已发表的 MIC 截断值。来自新鲜组织的比较分离株与来自同一组织的冷冻分离株具有相同的抗菌药物敏感性。本研究表明，即使在冷冻超过 10 年后，也可以从企鹅的冷冻呼吸道组织中分离出 。
