ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Barcelona, Spain.
Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), 28029 Madrid, Spain.
J Phys Chem Lett. 2021 Feb 4;12(4):1175-1181. doi: 10.1021/acs.jpclett.0c03439. Epub 2021 Jan 22.
Lateral compartmentalization of the plasma membrane is a prominent feature present at multiple spatiotemporal scales that regulates key cellular functions. The extracellular glycocalyx matrix has recently emerged as an important player that modulates the organization of specific receptors and patterns the lipid bilayer itself. However, experimental limitations in investigating its impact on the membrane nanoscale dynamics have hampered detailed studies. Here, we used photonic nanoantenna arrays combined with fluorescence correlation spectroscopy to investigate the influence of hyaluronic acid (HA), a prominent glycosaminoglycan, on the nanoscale organization of mimetic lipid bilayers. Using atomic force microscopy and force spectroscopy, we further correlated our dynamic measurements with the morphology and mechanical properties of bilayers at the nanoscale. Overall, we find that HA has a profound effect on the dynamics, nanoscale organization, and mechanical properties of lipid bilayers that are enriched in sphingolipids and/or cholesterol, such as those present in living cells.
质膜的侧向分隔是一种在多个时空尺度上表现突出的特征，它调节着关键的细胞功能。细胞外糖萼基质最近作为一种重要的调控因子出现，调节特定受体的组织和脂质双层本身的模式。然而，在研究其对膜纳米尺度动力学影响方面的实验限制阻碍了详细的研究。在这里，我们使用光子纳米天线阵列结合荧光相关光谱法来研究透明质酸（HA），一种重要的糖胺聚糖，对模拟脂质双层纳米尺度组织的影响。通过原子力显微镜和力谱学，我们进一步将我们的动态测量与纳米尺度上双层的形态和机械特性相关联。总的来说，我们发现 HA 对富含鞘脂和/或胆固醇的脂质双层的动力学、纳米尺度组织和机械特性有深远的影响，如存在于活细胞中的脂质双层。
